Reference - Detail
| RRC ID | 53789 |
|---|---|
| Author | Kadowaki T, Yukitake H, Naito M, Sato K, Kikuchi Y, Kondo Y, Shoji M, Nakayama K. |
| Title | A two-component system regulates gene expression of the type IX secretion component proteins via an ECF sigma factor. |
| Journal | Sci Rep |
| Abstract |
The periodontopathogen Porphyromonas gingivalis secretes potent pathogenic proteases, gingipains, via the type IX secretion system (T9SS). This system comprises at least 11 components; however, the regulatory mechanism of their expression has not yet been elucidated. Here, we found that the PorY (PGN_2001)-PorX (PGN_1019)-SigP (PGN_0274) cascade is involved in the regulation of T9SS. Surface plasmon resonance (SPR) analysis revealed a direct interaction between a recombinant PorY (rPorY) and a recombinant PorX (rPorX). rPorY autophosphorylated and transferred a phosphoryl group to rPorX in the presence of Mn(2+). These results demonstrate that PorX and PorY act as a response regulator and a histidine kinase, respectively, of a two component system (TCS), although they are separately encoded on the chromosome. T9SS component-encoding genes were down-regulated in a mutant deficient in a putative extracytoplasmic function (ECF) sigma factor, PGN_0274 (SigP), similar to the porX mutant. Electrophoretic gel shift assays showed that rSigP bound to the putative promoter regions of T9SS component-encoding genes. The SigP protein was lacking in the porX mutant. Co-immunoprecipitation and SPR analysis revealed the direct interaction between SigP and PorX. Together, these results indicate that the PorXY TCS regulates T9SS-mediated protein secretion via the SigP ECF sigma factor. |
| Volume | 6 |
| Pages | 23288 |
| Published | 2016-3-21 |
| DOI | 10.1038/srep23288 |
| PII | srep23288 |
| PMID | 26996145 |
| PMC | PMC4800418 |
| MeSH | Bacterial Proteins / chemistry Bacterial Proteins / genetics* Bacterial Proteins / metabolism Bacterial Secretion Systems / genetics* Bacterial Secretion Systems / metabolism Gene Expression Regulation, Bacterial Phosphorylation Porphyromonas gingivalis / genetics* Porphyromonas gingivalis / metabolism Promoter Regions, Genetic Protein Binding Protein Processing, Post-Translational Protein Transport Sigma Factor / physiology* Transcription, Genetic |
| IF | 3.998 |
| Times Cited | 28 |
| Altmetric score |
オルトメトリクス指標項目
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| Total number of mentions | 0 |
| Resource | |
| Prokaryotes E. coli | S17-1 BL21(DE3) |