RRC ID 53798
Author Kodama T, Takamatsu H, Asai K, Kobayashi K, Ogasawara N, Watabe K.
Title The Bacillus subtilis yaaH gene is transcribed by SigE RNA polymerase during sporulation, and its product is involved in germination of spores.
Journal J. Bacteriol.
Abstract The expression of 21 novel genes located in the region from dnaA to abrB of the Bacillus subtilis chromosome was analyzed. One of the genes, yaaH, had a predicted promoter sequence conserved among SigE-dependent genes. Northern blot analysis revealed that yaaH mRNA was first detected from 2 h after the cessation of logarithmic growth (T(2)) of sporulation in wild-type cells and in spoIIIG (SigG(-)) and spoIVCB (SigK(-)) mutants but not in spoIIAC (SigF(-)) and spoIIGAB (SigE(-)) mutants. The transcription start point was determined by primer extension analysis; the -10 and -35 regions are very similar to the consensus sequences recognized by SigE-containing RNA polymerase. A YaaH-His tag fusion encoded by a plasmid with a predicted promoter for the yaaH gene was produced from T(2) of sporulation in a B. subtilis transformant and extracted from mature spores, indicating that the yaaH gene product is a spore protein. Inactivation of the yaaH gene by insertion of an erythromycin resistance gene did not affect vegetative growth or spore resistance to heat, chloroform, and lysozyme. The germination of yaaH mutant spores in a mixture of L-asparagine, D-glucose, D-fructose, and potassium chloride was almost the same as that of wild-type spores, but the mutant spores were defective in L-alanine-stimulated germination. These results suggest that yaaH is a novel gene encoding a spore protein produced in the mother cell compartment from T(2) of sporulation and that it is required for the L-alanine-stimulated germination pathway.
Volume 181(15)
Pages 4584-91
Published 1999-8
PMID 10419957
PMC PMC103590
MeSH Amino Acid Sequence Bacillus subtilis / enzymology Bacillus subtilis / genetics Bacillus subtilis / physiology* Bacterial Proteins / biosynthesis Bacterial Proteins / genetics* Bacterial Proteins / metabolism* Base Sequence Chromosomes, Bacterial / genetics Consensus Sequence DNA-Directed RNA Polymerases / metabolism* Gene Expression Regulation, Bacterial* Genes, Bacterial* Genotype Molecular Sequence Data Promoter Regions, Genetic Recombinant Fusion Proteins / biosynthesis Sequence Alignment Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid Sigma Factor / metabolism* Spores, Bacterial Transcription, Genetic*
IF 3.219
Resource
Prokaryotes B. subtilis MBS600+B11:B53 MBS601 MBS602 MBS603 MBS604 MBS642 MBS643 MBS644 MBS645