RRC ID 53853
Author Fujioka T, Shimizu N, Yoshino K, Miyoshi H, Nakamura Y.
Title Establishment of induced pluripotent stem cells from human neonatal tissues.
Journal Hum Cell
Abstract Following the success in establishing human induced pluripotent stem (iPS) cells, research into various applications of the cells derived from human iPS cells has begun in earnest. The use of iPS cell-derived cells in clinical therapies is one of the most exciting of the possible applications. However, the risk of tumorigenicity is the biggest potential obstacle to use iPS cell derivatives in the clinic. It should be noted that the human cells used to generate iPS cell lines may have acquired genetic mutations and these might influence the tumorigenicity of the cells. In particular, the cells of older people have a higher risk of genetic mutations than those of younger people. Here, we show that iPS cells could be derived from short-term cultures of neonatal tissues. The established human iPS cells expressed various markers of undifferentiated cells and formed teratoma in immunodeficient mice. The human iPS cells derived from neonatal tissues may represent a clinical material possessing less tumorigenicity.
Volume 23(3)
Pages 113-8
Published 2010-8-1
DOI 10.1111/j.1749-0774.2010.00091.x
PMID 20973836
MeSH Animals Cell Line Cell Transformation, Neoplastic Extraembryonic Membranes / cytology* Humans Infant, Newborn Karyotyping Mice Microsatellite Repeats Neoplasm Transplantation Pluripotent Stem Cells* / cytology Pluripotent Stem Cells* / pathology Teratoma / pathology Umbilical Cord / cytology*
IF 2.333
Times Cited 16
DNA material pENTR/hKlf4 (RDB06433) pENTR/hOct3/4 (RDB06434) pENTR/hc-Myc (RDB06435) pENTR/hSox2 (RDB06436) CSII-EF-hOct3/4-IRES2-Venus (RDB12908) CSII-EF-hSox2-IRES2-Venus (RDB12909) CSII-EF-hKlf4-IRES2-Venus (RDB12910) CSII-EF-hc-Myc-IRES2-Venus (RDB12911)
Human and Animal Cells HUC-F2(RCB0436) HUC-Fm(RCB0197)