論文 - 詳細
| RRC ID | 53864 |
|---|---|
| 著者 | Tsuchiya M, Hara Y, Okuda M, Itoh K, Nishioka R, Shiomi A, Nagao K, Mori M, Mori Y, Ikenouchi J, Suzuki R, Tanaka M, Ohwada T, Aoki J, Kanagawa M, Toda T, Nagata Y, Matsuda R, Takayama Y, Tominaga M, Umeda M. |
| タイトル | Cell surface flip-flop of phosphatidylserine is critical for PIEZO1-mediated myotube formation. |
| ジャーナル | Nat Commun |
| Abstract |
Myotube formation by fusion of myoblasts and subsequent elongation of the syncytia is essential for skeletal muscle formation. However, molecules that regulate myotube formation remain elusive. Here we identify PIEZO1, a mechanosensitive Ca2+ channel, as a key regulator of myotube formation. During myotube formation, phosphatidylserine, a phospholipid that resides in the inner leaflet of the plasma membrane, is transiently exposed to cell surface and promotes myoblast fusion. We show that cell surface phosphatidylserine inhibits PIEZO1 and that the inward translocation of phosphatidylserine, which is driven by the phospholipid flippase complex of ATP11A and CDC50A, is required for PIEZO1 activation. PIEZO1-mediated Ca2+ influx promotes RhoA/ROCK-mediated actomyosin assemblies at the lateral cortex of myotubes, thus preventing uncontrolled fusion of myotubes and leading to polarized elongation during myotube formation. These results suggest that cell surface flip-flop of phosphatidylserine acts as a molecular switch for PIEZO1 activation that governs proper morphogenesis during myotube formation. |
| 巻・号 | 9(1) |
| ページ | 2049 |
| 公開日 | 2018-5-24 |
| DOI | 10.1038/s41467-018-04436-w |
| PII | 10.1038/s41467-018-04436-w |
| PMID | 29799007 |
| PMC | PMC5967302 |
| MeSH | Animals Calcium / metabolism Cell Differentiation* Cell Line Cell Membrane / genetics Cell Membrane / metabolism* Humans Ion Channels / genetics Ion Channels / metabolism* Mice Muscle Fibers, Skeletal / cytology Muscle Fibers, Skeletal / metabolism* Phosphatidylserines / metabolism* |
| IF | 12.121 |
| 引用数 | 23 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 24 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 293(RCB1637) |