論文 - 詳細
| RRC ID | 53867 |
|---|---|
| 著者 | Sumide K, Matsuoka Y, Kawamura H, Nakatsuka R, Fujioka T, Asano H, Takihara Y, Sonoda Y. |
| タイトル | A revised road map for the commitment of human cord blood CD34-negative hematopoietic stem cells. |
| ジャーナル | Nat Commun |
| Abstract |
We previously identified CD34-negative (CD34-) severe combined immunodeficiency (SCID)-repopulating cells as primitive hematopoietic stem cells (HSCs) in human cord blood. In this study, we develop a prospective ultra-high-resolution purification method by applying two positive markers, CD133 and GPI-80. Using this method, we succeed in purifying single long-term repopulating CD34- HSCs with self-renewing capability residing at the apex of the human HSC hierarchy from cord blood, as evidenced by a single-cell-initiated serial transplantation analysis. The gene expression profiles of individual CD34+ and CD34- HSCs and a global gene expression analysis demonstrate the unique molecular signature of CD34- HSCs. We find that the purified CD34- HSCs show a potent megakaryocyte/erythrocyte differentiation potential in vitro and in vivo. Megakaryocyte/erythrocyte progenitors may thus be generated directly via a bypass route from the CD34- HSCs. Based on these data, we propose a revised road map for the commitment of human CD34- HSCs in cord blood. |
| 巻・号 | 9(1) |
| ページ | 2202 |
| 公開日 | 2018-6-6 |
| DOI | 10.1038/s41467-018-04441-z |
| PII | 10.1038/s41467-018-04441-z |
| PMID | 29875383 |
| PMC | PMC5989201 |
| MeSH | Animals Antigens, CD34 / metabolism Cell Differentiation / genetics Cell Lineage / genetics Cell Self Renewal* Cell Separation / methods Fetal Blood / cytology* Gene Expression Profiling* Hematopoietic Stem Cell Transplantation / methods Hematopoietic Stem Cells / metabolism* Humans Mice, Inbred NOD Mice, Knockout Mice, SCID Prospective Studies Single-Cell Analysis / methods |
| IF | 12.121 |
| 引用数 | 5 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 9 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | THP-1(RCB1189) |