| RRC ID |
53867
|
| 著者 |
Sumide K, Matsuoka Y, Kawamura H, Nakatsuka R, Fujioka T, Asano H, Takihara Y, Sonoda Y.
|
| タイトル |
A revised road map for the commitment of human cord blood CD34-negative hematopoietic stem cells.
|
| ジャーナル |
Nat Commun
|
| Abstract |
We previously identified CD34-negative (CD34-) severe combined immunodeficiency (SCID)-repopulating cells as primitive hematopoietic stem cells (HSCs) in human cord blood. In this study, we develop a prospective ultra-high-resolution purification method by applying two positive markers, CD133 and GPI-80. Using this method, we succeed in purifying single long-term repopulating CD34- HSCs with self-renewing capability residing at the apex of the human HSC hierarchy from cord blood, as evidenced by a single-cell-initiated serial transplantation analysis. The gene expression profiles of individual CD34+ and CD34- HSCs and a global gene expression analysis demonstrate the unique molecular signature of CD34- HSCs. We find that the purified CD34- HSCs show a potent megakaryocyte/erythrocyte differentiation potential in vitro and in vivo. Megakaryocyte/erythrocyte progenitors may thus be generated directly via a bypass route from the CD34- HSCs. Based on these data, we propose a revised road map for the commitment of human CD34- HSCs in cord blood.
|
| 巻・号 |
9(1)
|
| ページ |
2202
|
| 公開日 |
2018-6-6
|
| DOI |
10.1038/s41467-018-04441-z
|
| PII |
10.1038/s41467-018-04441-z
|
| PMID |
29875383
|
| PMC |
PMC5989201
|
| MeSH |
Animals
Antigens, CD34 / metabolism
Cell Differentiation / genetics
Cell Lineage / genetics
Cell Self Renewal*
Cell Separation / methods
Fetal Blood / cytology*
Gene Expression Profiling*
Hematopoietic Stem Cell Transplantation / methods
Hematopoietic Stem Cells / metabolism*
Humans
Mice, Inbred NOD
Mice, Knockout
Mice, SCID
Prospective Studies
Single-Cell Analysis / methods
|
| IF |
12.121
|
| 引用数 |
5
|
| リソース情報 |
| ヒト・動物細胞 |
THP-1(RCB1189) |
