RRC ID 539
著者 Ote M, Mita K, Kawasaki H, Kobayashi M, Shimada T.
タイトル Characteristics of two genes encoding proteins with an ADAM-type metalloprotease domain, which are induced during the molting periods in Bombyx mori.
ジャーナル Arch Insect Biochem Physiol
Abstract By microarray analyses, we identified two genes (BmADAMTS-1 and BmADAMTS-like) encoding a protein, which are induced during the pupal ecdysis in the wing discs of Bombyx mori; these genes are homologous to ADAMTS family members (a disintegrin and metalloproteinase domain, with thrombospondin type-1 repeats). A complete metal-binding motif of the ADAM-type metalloprotease domain (HEXXHXXGXXHD) was contained in both amino acid sequences. However, thrombospondin type 1 (TSP-1) repeats were observed only in BmADAMTS-1. The BmADAMTS-1 gene was expressed in the hemocyte and midgut of the larvae at day 2 of wandering stage (W2), and strongly induced during the pupal ecdysis in the hemolymph. The BmADAMTS-like gene was expressed in the epithelial tissues of the larvae at W2, and had expression peaks slightly later than the BmADAMTS-1 gene. Our results indicate that BmADAMTS-1 and BmADAMTS-like may cleave the extracellular matrix (ECM) in the degenerating and remodeling tissues during the molting periods.
巻・号 59(2)
ページ 91-8
公開日 2005-6-1
DOI 10.1002/arch.20059
PMID 15898116
MeSH ADAM Proteins ADAMTS1 Protein Amino Acid Motifs / genetics Amino Acid Sequence Animals Base Sequence Blotting, Northern Bombyx / genetics* Bombyx / metabolism DNA Primers DNA, Complementary / genetics Disintegrins / biosynthesis* Disintegrins / genetics* Disintegrins / metabolism Enzyme Induction / genetics Extracellular Matrix Proteins / genetics* Extracellular Matrix Proteins / metabolism Gene Components Larva / metabolism Metalloendopeptidases / biosynthesis* Metalloendopeptidases / genetics* Metalloendopeptidases / metabolism Molecular Sequence Data Molting / genetics Molting / physiology* Oligonucleotide Array Sequence Analysis Reverse Transcriptase Polymerase Chain Reaction Sequence Analysis, DNA Thrombospondin 1 / genetics
IF 1.536
引用数 5
WOS 分野 ENTOMOLOGY PHYSIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
カイコ p50