| RRC ID |
53936
|
| Author |
Harvald EB, Sprenger RR, Dall KB, Ejsing CS, Nielsen R, Mandrup S, Murillo AB, Larance M, Gartner A, Lamond AI, Færgeman NJ.
|
| Title |
Multi-omics Analyses of Starvation Responses Reveal a Central Role for Lipoprotein Metabolism in Acute Starvation Survival in C. elegans.
|
| Journal |
Cell Syst
|
| Abstract |
Starvation causes comprehensive metabolic changes, which are still not fully understood. Here, we used quantitative proteomics and RNA sequencing to examine the temporal starvation responses in wild-type Caenorhabditis elegans and animals lacking the transcription factor HLH-30. Our findings show that starvation alters the abundance of hundreds of proteins and mRNAs in a temporal manner, many of which are involved in central metabolic pathways, including lipoprotein metabolism. We demonstrate that premature death of hlh-30 animals under starvation can be prevented by knockdown of either vit-1 or vit-5, encoding two different lipoproteins. We further show that the size and number of intestinal lipid droplets under starvation are altered in hlh-30 animals, which can be rescued by knockdown of vit-1. Taken together, this indicates that survival of hlh-30 animals under starvation is closely linked to regulation of intestinal lipid stores. We provide the most detailed poly-omic analysis of starvation responses to date, which serves as a resource for further mechanistic studies of starvation.
|
| Volume |
5(1)
|
| Pages |
38-52.e4
|
| Published |
2017-7-26
|
| DOI |
10.1016/j.cels.2017.06.004
|
| PII |
S2405-4712(17)30232-6
|
| PMID |
28734827
|
| MeSH |
Animals
Basic Helix-Loop-Helix Transcription Factors / genetics*
Caenorhabditis elegans / physiology*
Caenorhabditis elegans Proteins / genetics*
Gene Knockout Techniques
Lipid Droplets
Lipoproteins / metabolism*
Particle Size
Proteomics
RNA Interference
Sequence Analysis, RNA
Starvation / metabolism*
Vitellogenins / genetics
|
| IF |
8.673
|
| Times Cited |
13
|
| Resource |
| C.elegans |
tm1978 |
