RRC ID 53968
Author Chimalapati S, de Souza Santos M, Servage K, De Nisco NJ, Dalia AB, Orth K.
Title Natural Transformation in Vibrio parahaemolyticus: a Rapid Method To Create Genetic Deletions.
Journal J. Bacteriol.
Abstract The Gram-negative bacterium Vibrio parahaemolyticus is an opportunistic human pathogen and the leading cause of seafood-borne acute gastroenteritis worldwide. Recently, this bacterium was implicated as the etiologic agent of a severe shrimp disease with consequent devastating outcomes to shrimp farming. In both cases, acquisition of genetic material via horizontal transfer provided V. parahaemolyticus with new virulence tools to cause disease. Dissecting the molecular mechanisms of V. parahaemolyticus pathogenesis often requires manipulating its genome. Classically, genetic deletions in V. parahaemolyticus are performed using a laborious, lengthy, multistep process. Here, we describe a fast and efficient method to edit this bacterium's genome based on V. parahaemolyticus natural competence. Although this method is similar to one previously described, V. parahaemolyticus requires counterselection for curing of acquired plasmids due to its recalcitrant nature of retaining extrachromosomal DNA. We believe this approach will be of use to the Vibrio community.IMPORTANCE Spreading of vibrios throughout the world correlates with increased global temperatures. As they spread, they find new niches in which to survive, proliferate, and invade. Therefore, genetic manipulation of vibrios is of the utmost importance for studying these species. Here, we have delineated and validated a rapid method to create genetic deletions in Vibrio parahaemolyticus This study provides insightful methodology for studies with other Vibrio species.
Volume 200(15)
Published 2018-8-1
DOI 10.1128/JB.00032-18
PII JB.00032-18
PMID 29555695
PMC PMC6040184
IF 3.234
Times Cited 6
Resource
Pathogenic bacteria Vibrio parahaemolyticus