論文 - 詳細
| RRC ID | 53987 |
|---|---|
| 著者 | Saeki N, Saito A, Sugaya Y, Amemiya M, Sasaki H. |
| タイトル | Indirect Down-regulation of Tumor-suppressive let-7 Family MicroRNAs by LMO1 in Neuroblastoma. |
| ジャーナル | Cancer Genomics Proteomics |
| Abstract |
BACKGROUND/AIM:Overall survival for the high-risk group of neuroblastoma (NB) patients still remains at 40-50%, necessitating the establishment of a curable treatment. LIM domain only 1 (LMO1) gene encoding a transcriptional regulator is an NB-susceptibility gene with a tumor-promoting activity. Previously we conducted chromatin immunoprecipitation and DNA sequencing analyses on NB cell lines and identified 3 protein-coding genes regulated by LMO1. In this study, we extended our analyses to capture microRNA genes directly or indirectly regulated by LMO1. MATERIALS AND METHODS:Using microarrays, we conducted a comparative gene expression analysis on an NB cell line SK-N-SH; between the cells with and without LMO1 suppression. RESULTS:Overall, 18 microRNAs were identified to be indirectly down-regulated by LMO1 including 7 microRNAs of the let-7 family, whose cell proliferation inhibitory activity was observed. CONCLUSION:Target genes of the LMO1-regulated microRNAs and their relevant pathways may be a potential therapeutic target. |
| 巻・号 | 15(5) |
| ページ | 413-420 |
| 公開日 | 2018-1-1 |
| DOI | 10.21873/cgp.20100 |
| PII | 15/5/413 |
| PMID | 30194082 |
| PMC | PMC6199578 |
| MeSH | Cell Line, Tumor Cell Proliferation / genetics DNA-Binding Proteins / genetics* Gene Expression Regulation / genetics Humans LIM Domain Proteins / genetics* MicroRNAs / genetics* Microarray Analysis / methods Neuroblastoma / genetics* Neuroblastoma / pathology Transcription Factors / genetics* |
| IF | 3.147 |
| 引用数 | 1 |
| リソース情報 | |
| ヒト・動物細胞 | LA-N-5(RCB0485) |