RRC ID |
53996
|
著者 |
Matsuya Y, McMahon SJ, Tsutsumi K, Sasaki K, Okuyama G, Yoshii Y, Mori R, Oikawa J, Prise KM, Date H.
|
タイトル |
Investigation of dose-rate effects and cell-cycle distribution under protracted exposure to ionizing radiation for various dose-rates.
|
ジャーナル |
Sci Rep
|
Abstract |
During exposure to ionizing radiation, sub-lethal damage repair (SLDR) competes with DNA damage induction in cultured cells. By virtue of SLDR, cell survival increases with decrease of dose-rate, so-called dose-rate effects (DREs). Here, we focused on a wide dose-rate range and investigated the change of cell-cycle distribution during X-ray protracted exposure and dose-response curves via hybrid analysis with a combination of in vitro experiments and mathematical modelling. In the course of flow-cytometric cell-cycle analysis and clonogenic assays, we found the following responses in CHO-K1 cells: (1) The fraction of cells in S phase gradually increases during 6 h exposure at 3.0 Gy/h, which leads to radio-resistance. (2) Slight cell accumulation in S and G2/M phases is observed after exposure at 6.0 Gy/h for more than 10 hours. This suggests that an increase of SLDR rate for cells in S phase during irradiation may be a reproducible factor to describe changes in the dose-response curve at dose-rates of 3.0 and 6.0 Gy/h. By re-evaluating cell survival for various dose-rates of 0.186-60.0 Gy/h considering experimental-based DNA content and SLDR, it is suggested that the change of S phase fraction during irradiation modulates the dose-response curve and is possibly responsible for some inverse DREs.
|
巻・号 |
8(1)
|
ページ |
8287
|
公開日 |
2018-5-29
|
DOI |
10.1038/s41598-018-26556-5
|
PII |
10.1038/s41598-018-26556-5
|
PMID |
29844494
|
PMC |
PMC5974424
|
MeSH |
Animals
CHO Cells / radiation effects
Cell Cycle / radiation effects*
Cell Division
Cell Survival / radiation effects*
Cricetulus
DNA Damage / radiation effects
Dose-Response Relationship, Radiation*
Models, Biological
Radiation, Ionizing
S Phase
|
IF |
3.998
|
引用数 |
12
|
リソース情報 |
ヒト・動物細胞 |
CHO-K1(RCB0285) |