| RRC ID |
54003
|
| Author |
Ali MFZ, Yasin IA, Ohta T, Hashizume A, Ido A, Takahashi T, Miura C, Miura T.
|
| Title |
The silkrose of Bombyx mori effectively prevents vibriosis in penaeid prawns via the activation of innate immunity.
|
| Journal |
Sci Rep
|
| Abstract |
We previously identified novel bioactive polysaccharides from Bactrocera cucurbitae and Antheraea yamamai that activate innate immunity in RAW264 murine macrophages. However, in terms of potential applications in the cultivation of prawns, there were problems with the availability of these insects. However, we have now identified a polysaccharide from Bombyx mori that activates innate immunity in RAW264 cells and penaeid prawns. This purified polysaccharide, termed silkrose of B. mori (silkrose-BM), has a molecular weight of 1,150,000 and produces a single symmetrical peak on HPLC. Eight of nine constitutive monosaccharides of silkrose-BM are concomitant with dipterose of B. cucurbitae (dipterose-BC) and silkrose of A. yamamai (silkrose-AY). The major differences are found in the molar ratios of the monosaccharides. Silkrose-BM is approximately 500-fold less potent than silkrose-AY (EC50: 2.5 and 0.0043 μg/mL, respectively) in a nitrite oxide (NO) production assay using RAW264 cells. However, the maximum NO production for silkrose-BM and AY were comparable and higher than that of the lipopolysaccharide of Escherichia coli. The survival of penaeid prawns (Litopenaeus vannamei and Marsupenaeus japonicus) after infection with Vibrio penaecida was significantly improved by both dietary silkrose-BM and B. mori pupae. This suggests that silkrose-BM effectively prevents vibriosis in penaeid prawns via the activation of innate immunity.
|
| Volume |
8(1)
|
| Pages |
8836
|
| Published |
2018-6-11
|
| DOI |
10.1038/s41598-018-27241-3
|
| PII |
10.1038/s41598-018-27241-3
|
| PMID |
29892000
|
| PMC |
PMC5995915
|
| MeSH |
Animals
Aquaculture
Bombyx / chemistry*
Chromatography, High Pressure Liquid
Immunity, Innate / drug effects*
Immunologic Factors / administration & dosage
Immunologic Factors / chemistry
Immunologic Factors / isolation & purification*
Macrophages / drug effects
Macrophages / immunology
Mice
Molecular Weight
Nitric Oxide / metabolism
Penaeidae / growth & development*
Polysaccharides / administration & dosage
Polysaccharides / chemistry
Polysaccharides / isolation & purification*
RAW 264.7 Cells
Survival Analysis
Vibrio Infections / prevention & control
Vibrio Infections / veterinary*
|
| IF |
3.998
|
| Times Cited |
4
|
| Resource |
| Human and Animal Cells |
RAW 264(RCB0535) |
