論文 - 詳細
| RRC ID | 54003 |
|---|---|
| 著者 | Ali MFZ, Yasin IA, Ohta T, Hashizume A, Ido A, Takahashi T, Miura C, Miura T. |
| タイトル | The silkrose of Bombyx mori effectively prevents vibriosis in penaeid prawns via the activation of innate immunity. |
| ジャーナル | Sci Rep |
| Abstract |
We previously identified novel bioactive polysaccharides from Bactrocera cucurbitae and Antheraea yamamai that activate innate immunity in RAW264 murine macrophages. However, in terms of potential applications in the cultivation of prawns, there were problems with the availability of these insects. However, we have now identified a polysaccharide from Bombyx mori that activates innate immunity in RAW264 cells and penaeid prawns. This purified polysaccharide, termed silkrose of B. mori (silkrose-BM), has a molecular weight of 1,150,000 and produces a single symmetrical peak on HPLC. Eight of nine constitutive monosaccharides of silkrose-BM are concomitant with dipterose of B. cucurbitae (dipterose-BC) and silkrose of A. yamamai (silkrose-AY). The major differences are found in the molar ratios of the monosaccharides. Silkrose-BM is approximately 500-fold less potent than silkrose-AY (EC50: 2.5 and 0.0043 μg/mL, respectively) in a nitrite oxide (NO) production assay using RAW264 cells. However, the maximum NO production for silkrose-BM and AY were comparable and higher than that of the lipopolysaccharide of Escherichia coli. The survival of penaeid prawns (Litopenaeus vannamei and Marsupenaeus japonicus) after infection with Vibrio penaecida was significantly improved by both dietary silkrose-BM and B. mori pupae. This suggests that silkrose-BM effectively prevents vibriosis in penaeid prawns via the activation of innate immunity. |
| 巻・号 | 8(1) |
| ページ | 8836 |
| 公開日 | 2018-6-11 |
| DOI | 10.1038/s41598-018-27241-3 |
| PII | 10.1038/s41598-018-27241-3 |
| PMID | 29892000 |
| PMC | PMC5995915 |
| MeSH | Animals Aquaculture Bombyx / chemistry* Chromatography, High Pressure Liquid Immunity, Innate / drug effects* Immunologic Factors / administration & dosage Immunologic Factors / chemistry Immunologic Factors / isolation & purification* Macrophages / drug effects Macrophages / immunology Mice Molecular Weight Nitric Oxide / metabolism Penaeidae / growth & development* Polysaccharides / administration & dosage Polysaccharides / chemistry Polysaccharides / isolation & purification* RAW 264.7 Cells Survival Analysis Vibrio Infections / prevention & control Vibrio Infections / veterinary* |
| IF | 3.998 |
| 引用数 | 4 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |