論文 - 詳細
| RRC ID | 54017 |
|---|---|
| 著者 | Tasaka T, Kuwada E, Izuchi Y, Nishigawa R, Yamada H, Unuma H, Tokunaga K, Hayakawa A, Go A, Go K, Uto Y. |
| タイトル | Concentration-dependent Activation of Inflammatory/Anti-inflammatory Functions of Macrophages by Hydrolyzed Whey Protein. |
| ジャーナル | Anticancer Res |
| Abstract |
BACKGROUND/AIM:Whey protein is a mixture of globulins isolated from whey and mainly composed of β-lactoglobulin, α-lactoalbumin, and lactoferrin. In this study, whey protein was hydrolyzed using various proteases, and the macrophage activation was evaluated. MATERIALS AND METHODS:Hydrolyzed whey protein was prepared using various proteases to evaluate phagocytic activity and cytokine productivity. RESULTS:The results of SDS-PAGE and gel permeation chromatography (GPC) analysis indicated that the molecular weight of whey protein was reduced using various proteases. The hydrolyzed whey protein showed a concentration-dependent induction of macrophage phagocytic activity. In addition, the hydrolyzed whey protein significantly enhanced the production of the inflammatory cytokine, TNF-α. Production of the anti-inflammatory cytokine, IL-10, was not observed at concentrations up to 1 μg, but significant production was confirmed at 100 μg. CONCLUSION:Hydrolyzed whey protein can induce the phagocytic activity of macrophages and activation of the inflammatory/anti-inflammatory functions of the macrophages depends on the concentration of the hydrolyzed whey protein. |
| 巻・号 | 38(7) |
| ページ | 4299-4304 |
| 公開日 | 2018-7-1 |
| DOI | 10.21873/anticanres.12728 |
| PII | 38/7/4299 |
| PMID | 29970565 |
| MeSH | Animals Anti-Inflammatory Agents / pharmacology Female Inflammation / immunology Macrophage Activation / drug effects* Macrophages / drug effects* Macrophages / immunology* Mice Mice, Inbred ICR Whey Proteins / pharmacology* |
| IF | 1.994 |
| 引用数 | 2 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |