| RRC ID |
54025
|
| Author |
Sakoda M, Kaneko M, Ohta S, Qi P, Ichimura S, Yatomi Y, Ito T.
|
| Title |
Injectable Hemostat Composed of a Polyphosphate-Conjugated Hyaluronan Hydrogel.
|
| Journal |
Biomacromolecules
|
| Abstract |
We have developed a new hydrogel hemostat composed of hyaluronan (HA) conjugated with inorganic polyphosphate (PolyP). A hemostatic hydrogel, HAX-PolyP, was formed rapidly by mixing aldehyde-modified HA and hydrazide-modified HA conjugated with PolyP (HA-PolyP). Although the gelation rate decreased with increasing PolyP content, the gelation time was below 5 min. In addition, the hydrogel swelling volume decreased with increasing PolyP content, but the degradation rate did not depend on PolyP content and the hydrogel underwent complete degradation through hydrolysis over 3 weeks in phosphate buffered saline. HAX-PolyP showed similar biocompatibility with the HA hydrogel without PolyP conjugation in vitro and in vivo. Intraperitoneal administration of HAX-PolyP did not induce any adhesion in the peritoneum and clot formation in the lungs. Finally, HA-PolyP accelerated the coagulation rate of human plasma ex vivo, and HAX-PolyP showed as strong a hemostatic effect as fibrin glue in a mouse liver bleeding model in vivo.
|
| Volume |
19(8)
|
| Pages |
3280-3290
|
| Published |
2018-8-13
|
| DOI |
10.1021/acs.biomac.8b00588
|
| PMID |
29985587
|
| MeSH |
3T3 Cells
Animals
Hemostatics / administration & dosage
Hemostatics / chemistry*
Humans
Hyaluronic Acid / chemistry*
Hydrogels / chemistry*
Injections
Male
Mice
Mice, Inbred ICR
Polyphosphates / chemistry*
RAW 264.7 Cells
|
| IF |
6.092
|
| Times Cited |
5
|
| Resource |
| Human and Animal Cells |
RAW 264(RCB0535) |
