論文 - 詳細
| RRC ID | 54028 |
|---|---|
| 著者 | Kawai K, Kamochi R, Oiki S, Murata K, Hashimoto W. |
| タイトル | Probiotics in human gut microbiota can degrade host glycosaminoglycans. |
| ジャーナル | Sci Rep |
| Abstract |
Glycosaminoglycans (GAGs) (e.g. heparin, chondroitin sulfate, and hyaluronan) show various significant physiological functions as a major component of extracellular matrix in animals. Some bacteria target GAGs for adhesion and/or infection to host cells, although no probiotics have been known to degrade GAGs. Here, we show GAG degradation by probiotics from human gut microbiota and their adhesion to human intestinal cells through a GAG. GAG-degrading bacteria were isolated from human faeces and identified as Enterococcus faecium, and some typical probiotics such as Lactobacillus casei, Lactobacillus rhamnosus and Enterococcus faecalis were also found to degrade heparin. GAG-degrading lactobacilli and enterococci including the isolated E. faecium possessed a genetic cluster encoding GAG-degrading/metabolising enzymes in the bacterial genome. KduI and KduD enzymes encoded in the GAG cluster of L. rhamnosus functioned as 4-deoxy-l-threo-5-hexosulose-uronate ketol-isomerase and 2-keto-3-deoxy-d-gluconate dehydrogenase, respectively, both of which were crucial for GAG metabolism. GAG-degrading L. rhamnosus and E. faecium attached to human intestinal Caco-2 cells via heparin. Some species of Bacteroides, considered to be the next generation probiotics, degraded chondroitin sulfate C and hyaluronan, and genes coding for the Bacteroides GAG-degrading enzyme were frequently detected from human gut microbiota. This is the first report on GAG-degrading probiotics in human gut microbiota. |
| 巻・号 | 8(1) |
| ページ | 10674 |
| 公開日 | 2018-7-13 |
| DOI | 10.1038/s41598-018-28886-w |
| PII | 10.1038/s41598-018-28886-w |
| PMID | 30006634 |
| PMC | PMC6045597 |
| MeSH | Aldose-Ketose Isomerases / genetics Aldose-Ketose Isomerases / metabolism Bacteria / enzymology* Bacteria / genetics Bacterial Proteins / genetics Bacterial Proteins / isolation & purification Bacterial Proteins / metabolism* Caco-2 Cells Carbohydrate Dehydrogenases / genetics Carbohydrate Dehydrogenases / metabolism Coculture Techniques Feces / microbiology Gastrointestinal Microbiome / physiology* Glycosaminoglycans / metabolism* Humans Metagenomics Probiotics / metabolism* |
| IF | 3.998 |
| 引用数 | 7 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 15 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | CACO-2(RCB0988) |
| 一般微生物 | JCM 16067 JCM 13471 JCM 6297 JCM 13265 JCM 5824 JCM 15092 JCM 13657 JCM 9496 JCM 5827 JCM 5828 |