論文 - 詳細
| RRC ID | 54032 |
|---|---|
| 著者 | Haraguchi Y, Matsuura K, Kagawa Y, Hasegawa A, Kubo H, Shimizu T. |
| タイトル | Rapid creation system of morphologically and functionally communicative three-dimensional cell-dense tissue by centrifugation. |
| ジャーナル | Biotechnol Prog |
| Abstract |
This study reports a rapid fabrication system of a morphologically and functionally communicative three-dimensional (3D) cell-dense tissue without scaffolds by centrifugation. The tight adhesion between C2C12 myoblasts and culture surface was accelerated without significant cell damage by centrifugation (80 x g, 37 °C, 30 min). A thicker tissue created on a temperature-responsive culture surface was harvested by decreasing temperature. The 3D myoblast tissues having approximately 200 μm-thickness were created at 1.5 h [centrifugation (80 x g, 37 °C) for 30 min and tissue harvest for 1 h]. However, in the case of without centrifugation, the myoblast tissues had fragile parts even at 7.5 h after the incubation. Additionally, electrically/functionally communicative and thicker human induced pluripotent stem (iPS) cell-derived cardiac tissues were created rapidly by the centrifugation and cultivation at 37 °C. We report a centrifugation system that significantly shortens the creation time of 3D tissues. We envision that this procedure will contribute to the field of tissue engineering and regenerative medicine. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 34:1447-1453, 2018. |
| 巻・号 | 34(6) |
| ページ | 1447-1453 |
| 公開日 | 2018-11-1 |
| DOI | 10.1002/btpr.2691 |
| PMID | 30009512 |
| MeSH | Cell Culture Techniques / methods* Humans Induced Pluripotent Stem Cells / cytology* Myoblasts / cytology Tissue Engineering / methods* |
| IF | 2.334 |
| 引用数 | 1 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 201B7(HPS0063) |