RRC ID 54036
著者 Mohara M, Kawasaki T, Owada R, Imai T, Kanetaka H, Izumi SI, Tsukiyama K, Nakamura K.
タイトル Restoration from polyglutamine toxicity after free electron laser irradiation of neuron-like cells.
ジャーナル Neurosci Lett
Abstract Proteins containing an expanded polyglutamine tract tend to aggregate, leading to the neuronal damage observed in polyglutamine diseases. We recently reported that free electron laser (FEL) irradiation markedly dissociates naked polyglutamine aggregates as well as the aggregate in the 293 T cells. In the present study, we investigated whether FEL irradiation of neuron-like cells with polyglutamine aggregates would restore the cellular damage and dysfunction. The aggregated polyglutamine peptides induced neurite retraction of differentiated SH-SY5Y cells. Upon FEL irradiation, the polyglutamine aggregates in the SH-SY5Y cells were dissociated, and the shorter length of individual neurite, fewer number of neurites per cell and shorter total length of neurite by polyglutamine were inhibited. Same results were essentially obtained in PC12 cells. Moreover, when FEL irradiation was applied to undifferentiated SH-SY5Y cells, the deficits in neuron-like differentiation seen in expanded polyglutamine peptide-containing cells were also rescued. Thus, FEL irradiation restored both the damage and differentiation caused by polyglutamine in neuron-like cells.
巻・号 685
ページ 42-49
公開日 2018-10-15
DOI 10.1016/j.neulet.2018.07.031
PII S0304-3940(18)30504-4
PMID 30044955
MeSH Animals Cell Differentiation / drug effects Cell Differentiation / physiology Electrons / therapeutic use* Humans Lasers* Neurites / drug effects Neurites / metabolism Neuroblastoma / drug therapy Neuroblastoma / metabolism Neurons / drug effects* Neurons / metabolism PC12 Cells Peptides / drug effects Peptides / metabolism Peptides / toxicity* Rats
IF 2.274
引用数 0
リソース情報
ヒト・動物細胞 PC-12(RCB0009)