論文 - 詳細
| RRC ID | 54038 |
|---|---|
| 著者 | Funakohi-Tago M, Sakata T, Fujiwara S, Sakakura A, Sugai T, Tago K, Tamura H. |
| タイトル | Hydroxytyrosol butyrate inhibits 6-OHDA-induced apoptosis through activation of the Nrf2/HO-1 axis in SH-SY5Y cells. |
| ジャーナル | Eur J Pharmacol |
| Abstract |
Hydroxytyrosol (HT) is a polyphenol contained in olives and exhibits antioxidant activity. We herein investigated the effects of HT and its derivatives, hydroxytyrosol acetate (HT-A) and hydroxytyrosol butyrate (HT-B), on the protection of neuronal cells against apoptosis induced by the Parkinson's disease-related neurotoxin 6-hydroxydopamine (6-OHDA). The pretreatment of SH-SY5Y cells with HT-B, but not HT or HT-A significantly reduced the 6-OHDA-induced generation of reactive oxygen species, activation of caspase-3, and subsequent cell death. HT-B also induced the protein expression of the transcription factor, NF-E2-related factor-2 (Nrf2) and its transcriptional activation, resulting in the up-regulated expression of heme oxygenase-1 (HO-1), which conferred neuroprotection against 6-OHDA-induced oxidative damage. Furthermore, three cysteine residues, Cys151, Cys273, and Cys288 in Kelch-like ECH-associated protein 1 (Keap1) were necessary for the HT-B-induced activation of Nrf2. Collectively, the present results demonstrated that HT-B, harboring higher fat solubility than HT and HT-A, effectively elicited adaptive responses to oxidative stress by activating the Nrf2/HO-1 axis in neuronal cells. |
| 巻・号 | 834 |
| ページ | 246-256 |
| 公開日 | 2018-9-5 |
| DOI | 10.1016/j.ejphar.2018.07.043 |
| PII | S0014-2999(18)30420-5 |
| PMID | 30053409 |
| MeSH | Apoptosis / drug effects* Cell Line, Tumor Gene Expression Regulation / drug effects Heme Oxygenase-1 / genetics Heme Oxygenase-1 / metabolism* Humans Kelch-Like ECH-Associated Protein 1 / chemistry Kelch-Like ECH-Associated Protein 1 / metabolism NF-E2-Related Factor 2 / genetics NF-E2-Related Factor 2 / metabolism* Neuroprotective Agents / chemistry Neuroprotective Agents / pharmacology* Oxidopamine / pharmacology* Phenylethyl Alcohol / analogs & derivatives* Phenylethyl Alcohol / chemistry Phenylethyl Alcohol / pharmacology RNA, Messenger / genetics RNA, Messenger / metabolism Transcriptional Activation / drug effects |
| IF | 3.263 |
| 引用数 | 17 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 293T(RCB2202) |