RRC ID 54041
Author Kuroda M, Ueda K, Kioka N.
Title Vinexin family (SORBS) proteins regulate mechanotransduction in mesenchymal stem cells.
Journal Sci Rep
Abstract The stiffness of extracellular matrix (ECM) directs the differentiation of mesenchymal stem cells (MSCs) through the transcriptional co-activators Yes-associated protein (YAP) and transcriptional coactivator with a PDZ-binding motif (TAZ). Although a recent study revealed the involvement of vinexin α and CAP (c-Cbl-associated proteins), two of vinexin (SORBS) family proteins that bind to vinculin, in mechanosensing, it is still unclear whether these proteins regulate mechanotransduction and differentiation of MSCs. In the present study, we show that both vinexin α and CAP are necessary for the association of vinculin with the cytoskeleton and the promotion of YAP/TAZ nuclear localization in MSCs grown on rigid substrates. Furthermore, CAP is involved in the MSC differentiation in a stiffness-dependent manner, whereas vinexin depletion suppresses adipocyte differentiation independently of YAP/TAZ. These observations reveal a critical role of vinexin α and CAP in mechanotransduction and MSC differentiation.
Volume 8(1)
Pages 11581
Published 2018-8-1
DOI 10.1038/s41598-018-29700-3
PII 10.1038/s41598-018-29700-3
PMID 30068914
PMC PMC6070524
MeSH Adaptor Proteins, Signal Transducing / metabolism Animals Cell Cycle Proteins Cell Differentiation* Cell Line Gene Expression Regulation* Mechanotransduction, Cellular* Mesenchymal Stem Cells / physiology* Mice Microfilament Proteins / metabolism* Muscle Proteins / metabolism* Phosphoproteins / metabolism Protein Binding Signal Transduction* Transcription Factors / metabolism Vinculin / metabolism
IF 4.011
Times Cited 3
Human and Animal Cells ST2(RCB0224)