論文 - 詳細
| RRC ID | 54042 |
|---|---|
| 著者 | Fujioka-Kobayashi M, Tsuru K, Nagai H, Fujisawa K, Kudoh T, Ohe G, Ishikawa K, Miyamoto Y. |
| タイトル | Fabrication and evaluation of carbonate apatite-coated calcium carbonate bone substitutes for bone tissue engineering. |
| ジャーナル | J Tissue Eng Regen Med |
| Abstract |
Carbonate apatite-coated calcium carbonate (CO3 Ap/CaCO3 ) was fabricated through a dissolution-precipitation reaction using CaCO3 granules as a precursor to accelerate bone replacement based on superior osteoconductivity of the CO3 Ap shell, along with Ca2+ release from the CaCO3 core and quicker resorption of the CaCO3 core. In the present study, CaCO3 , 10% CO3 Ap/CaCO3 , 30% CO3 Ap/CaCO3 , and CO3 Ap granules were fabricated and examined histologically to evaluate their potential as bone substitutes. Larger contents of CaCO3 in the granules resulted in higher Ca2+ release and promoted cell proliferation of murine preosteoblasts at 6 days compared with CO3 Ap. Interestingly, in a rabbit femur defect model, 10% CO3 Ap/CaCO3 induced significantly higher new bone formation and higher material resorption compared with CO3 Ap at 8 weeks. Nevertheless, CO3 Ap showed a superior osteoconductive potential compared with 10% CO3 Ap/CaCO3 at 8 weeks. All tested granules were most likely resorbed by cell mediation including multinucleated giant cell functions. Therefore, we conclude that CO3 Ap/CaCO3 has a positive potential for bone tissue engineering based on well-controlled calcium release, bone formation, and material resorption. |
| 巻・号 | 12(10) |
| ページ | 2077-2087 |
| 公開日 | 2018-10-1 |
| DOI | 10.1002/term.2742 |
| PMID | 30058260 |
| MeSH | Animals Apatites / pharmacology* Body Fluids / metabolism Bone Regeneration / drug effects Bone Substitutes / pharmacology* Bone and Bones / drug effects Bone and Bones / physiology* Calcium Carbonate / pharmacology* Cell Line Cell Proliferation / drug effects Coated Materials, Biocompatible / pharmacology* Femur / drug effects Femur / pathology Kinetics Male Mice Osteogenesis / drug effects Rabbits Tissue Engineering / methods* X-Ray Diffraction |
| IF | 3.078 |
| 引用数 | 4 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |