論文 - 詳細
| RRC ID | 54043 |
|---|---|
| 著者 | Kamei H, Noguchi K, Matsuda H, Murata K. |
| タイトル | Screening of Euphorbiaceae Plant Extracts for Anti-5α-reductase. |
| ジャーナル | Biol Pharm Bull |
| Abstract |
In our research program to find novel agents for alopecia from natural plant resources, we screened Euphorbiaceae plant extracts using an anti-5α-reductase assay. Among the samples tested, the extract of Phyllanthus urinaria showed the most potent activity with 24.3 and 64.6% inhibition at 50 and 200 µg/mL against the enzyme, respectively. The extract also suppressed the androgen activity of dihydrotestosterone in LNCaP cell line. These results show that the extract of P. urinaria may be a multi-potent agent for androgen-derived alopecia. We tested for activity on a hair regrowth model using mice. The extract of P. urinaria showed hair regrowth activity at 5 mg/mouse/d administration. Furthermore, the active principle for anti-5α-reductase activity was determined as stigmasterol glucoside from activity-guided fractionation and the IC50 was 27.2 µM. These results suggest that extract of P. urinaria may be a promising candidate anti-alopecia agent. |
| 巻・号 | 41(8) |
| ページ | 1307-1310 |
| 公開日 | 2018-1-1 |
| DOI | 10.1248/bpb.b18-00283 |
| PMID | 30068884 |
| MeSH | 5-alpha Reductase Inhibitors / pharmacology* Alopecia / drug therapy Androgens / metabolism Animals Cell Line Cholestenone 5 alpha-Reductase Dihydrotestosterone / metabolism Euphorbiaceae* Hair / drug effects* Hair / growth & development Male Mice, Inbred C57BL Plant Extracts / pharmacology* |
| IF | 1.863 |
| 引用数 | 1 |
| リソース情報 | |
| ヒト・動物細胞 | LNCap.FGC(RCB2144) |