論文 - 詳細
| RRC ID | 54050 |
|---|---|
| 著者 | Matsumoto S, Caliskan N, Rodnina MV, Murata A, Nakatani K. |
| タイトル | Small synthetic molecule-stabilized RNA pseudoknot as an activator for -1 ribosomal frameshifting. |
| ジャーナル | Nucleic Acids Res |
| Abstract |
Programmed -1 ribosomal frameshifting (-1PRF) is a recoding mechanism to make alternative proteins from a single mRNA transcript. -1PRF is stimulated by cis-acting signals in mRNA, a seven-nucleotide slippery sequence and a downstream secondary structure element, which is often a pseudoknot. In this study we engineered the frameshifting pseudoknot from the mouse mammary tumor virus to respond to a rationally designed small molecule naphthyridine carbamate tetramer (NCTn). We demonstrate that NCTn can stabilize the pseudoknot structure in mRNA and activate -1PRF both in vitro and in human cells. The results illustrate how NCTn-inducible -1PRF may serve as an important component of the synthetic biology toolbox for the precise control of gene expression using small synthetic molecules. |
| 巻・号 | 46(16) |
| ページ | 8079-8089 |
| 公開日 | 2018-9-19 |
| DOI | 10.1093/nar/gky689 |
| PII | 5063821 |
| PMID | 30085309 |
| PMC | PMC6144811 |
| MeSH | Animals Base Sequence / genetics Carbamates / chemical synthesis Carbamates / chemistry Frameshifting, Ribosomal / genetics* Gene Expression Regulation / drug effects* Mammary Tumor Virus, Mouse / genetics Mice Naphthyridines / chemical synthesis Naphthyridines / chemistry Nucleic Acid Conformation / drug effects Protein Biosynthesis / drug effects Protein Biosynthesis / genetics Protein Isoforms / genetics RNA / chemistry RNA / drug effects* RNA, Messenger / genetics RNA, Viral / genetics Small Molecule Libraries / chemical synthesis Small Molecule Libraries / chemistry Small Molecule Libraries / pharmacology* Synthetic Biology |
| IF | 11.502 |
| 引用数 | 7 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa(RCB0007) 293(RCB1637) |