論文 - 詳細
| RRC ID | 54057 |
|---|---|
| 著者 | Yamada S, Kubo Y, Yamazaki D, Sekino Y, Nomura Y, Yoshida S, Kanda Y. |
| タイトル | Tributyltin Inhibits Neural Induction of Human Induced Pluripotent Stem Cells. |
| ジャーナル | Sci Rep |
| Abstract |
Tributyltin (TBT), one of the organotin compounds, is a well-known environmental pollutant. In our recent study, we reported that TBT induces mitochondrial dysfunction, in human-induced pluripotent stem cells (iPSCs) through the degradation of mitofusin1 (Mfn1), which is a mitochondrial fusion factor. However, the effect of TBT toxicity on the developmental process of iPSCs was not clear. The present study examined the effect of TBT on the differentiation of iPSCs into the ectodermal, mesodermal, and endodermal germ layers. We found that exposure to nanomolar concentration of TBT (50 nM) selectively inhibited the induction of iPSCs into the ectoderm, which is the first step in neurogenesis. We further assessed the effect of TBT on neural differentiation and found that it reduced the expression of several neural differentiation marker genes, which were also downregulated by Mfn1 knockdown in iPSCs. Taken together, these results indicate that TBT induces developmental neurotoxicity via Mfn1-mediated mitochondrial dysfunction in iPSCs. |
| 巻・号 | 8(1) |
| ページ | 12155 |
| 公開日 | 2018-8-14 |
| DOI | 10.1038/s41598-018-30615-2 |
| PII | 10.1038/s41598-018-30615-2 |
| PMID | 30108368 |
| PMC | PMC6092327 |
| MeSH | Cell Differentiation / drug effects* Cell Line Environmental Pollutants / toxicity* GTP Phosphohydrolases / metabolism Humans Induced Pluripotent Stem Cells / cytology Induced Pluripotent Stem Cells / drug effects* Induced Pluripotent Stem Cells / physiology Mitochondria / drug effects Mitochondria / metabolism Mitochondrial Dynamics / drug effects Mitochondrial Membrane Transport Proteins / metabolism Neurogenesis / drug effects* Toxicity Tests Trialkyltin Compounds / toxicity* |
| IF | 3.998 |
| 引用数 | 0 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 7 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 253G1(HPS0002) |