Reference - Detail
| RRC ID | 54060 |
|---|---|
| Author | Nishioka K, Miyazaki H, Soejima H. |
| Title | Unbiased shRNA screening, using a combination of FACS and high-throughput sequencing, enables identification of novel modifiers of Polycomb silencing. |
| Journal | Sci Rep |
| Abstract |
Polycomb silencing is an important and rapidly growing field that is relevant to a broad range of aspects of human health, including cancer and stem cell biology. To date, the regulatory mechanisms for the fine-tuning of Polycomb silencing remain unclear, but it is likely that there is a series of unidentified factors that functionally modify or balance the silencing. However, a practical gene screening strategy for identifying such factors has not yet been developed. The failure of screening strategies used thus far is probably due to the effect of the loss-of-function phenotypes of these factors on cell cycle progression. Here, by applying fluorescence-activated cell sorter (FACS) and high-throughput sequencing (HTS) technology in a large-scale lentivirus-mediated shRNA screening, we obtained a consecutive dataset from all shRNAs tested, which highlighted a substantial number of genes that may control Polycomb silencing. We consider that this unbiased strategy can readily be applied to a wide range of studies to uncover novel regulatory layers for expression of genes of interest. |
| Volume | 8(1) |
| Pages | 12128 |
| Published | 2018-8-14 |
| DOI | 10.1038/s41598-018-30649-6 |
| PII | 10.1038/s41598-018-30649-6 |
| PMID | 30108332 |
| PMC | PMC6092423 |
| MeSH | Animals Cell Line, Tumor Cell Separation / methods Datasets as Topic Flow Cytometry / methods Genes, Reporter / genetics Genetic Vectors / genetics High-Throughput Nucleotide Sequencing / methods High-Throughput Screening Assays / methods* Lentivirus / genetics Mice Mouse Embryonic Stem Cells Polycomb-Group Proteins / genetics* RNA Interference RNA, Small Interfering / metabolism* |
| IF | 3.998 |
| Times Cited | 4 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 9 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | F9(RCB1555) |