RRC ID 54075
Author Kawasaki K, Okuda-Hanafusa C, Aoyagi M, Taoka K, Yamamoto N, Muroyama K, Murosaki S, Yamamoto Y.
Title Inhibitory effect of the compounds from the water extract of Curcuma longa on the production of PGE2 and NO in a macrophage cell line stimulated by LPS.
Journal Biosci Biotechnol Biochem
Abstract We wished to search for the compounds contributing to the anti-inflammatory effects of the water extract of Curcuma longa (WEC). WEC was fractioned and the fractions were evaluated with regard to their inhibitory effect on the production of nitric oxide (NO) from the macrophage cell line stimulated by lipopolysaccharide. Compounds in the active fractions were isolated and identified. One isolated compound was identified as new: (6S)-2-methyl-5-hydroxy-6-(3-hydroxy-4-methylphenyl)-2-heptene-4-one (1). Four isolated compounds were identified as known: (6S)-2-methyl-6-(4-hydroxyphenyl)-2-heptene-4-one (4), bisabolone-4-one (5), curcumenone (6), and turmeronol A (8). Three isolated compounds were not identified their stereostructures but their planar structures: 2-methyl-6-(4-hydroxymethyl-phenyl)-2-heptene-4-one (2), 2-methyl-6-(2,3-epoxy-4-methyl-4-cyclohexene)-2-heptene (3), and 4-methylene-5-hydroxybisabola-2,10-diene-9-one (7). Compounds 1, 4, 7 and 8 inhibited production of prostaglandin E2 and NO. Others inhibited NO production only. These results (at least in part) show the active compounds contributing to the anti-inflammatory effects of WEC, and may be useful for elucidating its various beneficial physiologic effects.
Volume 82(12)
Pages 2109-2117
Published 2018-12
DOI 10.1080/09168451.2018.1511366
PMID 30146952
MeSH Animals Anti-Inflammatory Agents / chemistry Anti-Inflammatory Agents / isolation & purification Anti-Inflammatory Agents / pharmacology* Carbon-13 Magnetic Resonance Spectroscopy Chromatography, High Pressure Liquid Curcuma / chemistry* Dinoprostone / biosynthesis* Lipopolysaccharides / pharmacology* Macrophages / drug effects* Macrophages / metabolism Mass Spectrometry / methods Mice Nitric Oxide / biosynthesis* Plant Extracts / chemistry Plant Extracts / isolation & purification Plant Extracts / pharmacology* Proton Magnetic Resonance Spectroscopy RAW 264.7 Cells Water
IF 1.297
Times Cited 5
Human and Animal Cells RAW 264(RCB0535)