Reference - Detail
| RRC ID | 54127 |
|---|---|
| Author | Mogi K, Hayashida K, Yamamoto T. |
| Title | Damage-less Handling of Exosomes Using an Ion-depletion Zone in a Microchannel. |
| Journal | Anal Sci |
| Abstract |
Exosomes are of increasing research interest because they are integral to cell-cell communication and are implicated in various disease states. Here, we investigated the utility of using an ion-depletion zone in a microfluidic device to concentrate exosomes from the culture media of four types of cell lines. Furthermore, we evaluated the extent of damage to the exosomes following concentration by an ion-depletion zone microchannel device compared with exosomes concentrated by a conventional ultra-centrifugation technique. Our results conclusively demonstrate that significantly less damage is incurred by exosomes following passage through and concentration by the ion-depleted zone microchannel device compared to concentration by ultra-centrifugation. Our findings will help extend the utility of exosomes to various applications. |
| Volume | 34(8) |
| Pages | 875-880 |
| Published | 2018-1-1 |
| DOI | 10.2116/analsci.17P462 |
| PMID | 30101880 |
| MeSH | Cell Communication Cell Line Centrifugation Culture Media / chemistry* Exosomes / chemistry* Humans Ions / chemistry Microfluidic Analytical Techniques* |
| IF | 2.049 |
| Times Cited | 3 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | MCF7(RCB1904) 293(RCB1637) A549(RCB0098) |