| RRC ID |
54127
|
| Author |
Mogi K, Hayashida K, Yamamoto T.
|
| Title |
Damage-less Handling of Exosomes Using an Ion-depletion Zone in a Microchannel.
|
| Journal |
Anal Sci
|
| Abstract |
Exosomes are of increasing research interest because they are integral to cell-cell communication and are implicated in various disease states. Here, we investigated the utility of using an ion-depletion zone in a microfluidic device to concentrate exosomes from the culture media of four types of cell lines. Furthermore, we evaluated the extent of damage to the exosomes following concentration by an ion-depletion zone microchannel device compared with exosomes concentrated by a conventional ultra-centrifugation technique. Our results conclusively demonstrate that significantly less damage is incurred by exosomes following passage through and concentration by the ion-depleted zone microchannel device compared to concentration by ultra-centrifugation. Our findings will help extend the utility of exosomes to various applications.
|
| Volume |
34(8)
|
| Pages |
875-880
|
| Published |
2018-1-1
|
| DOI |
10.2116/analsci.17P462
|
| PMID |
30101880
|
| MeSH |
Cell Communication
Cell Line
Centrifugation
Culture Media / chemistry*
Exosomes / chemistry*
Humans
Ions / chemistry
Microfluidic Analytical Techniques*
|
| IF |
2.049
|
| Times Cited |
3
|
| Resource |
| Human and Animal Cells |
MCF7(RCB1904)
293(RCB1637)
A549(RCB0098) |
