RRC ID 54130
著者 Miyaguchi J, Shiga K, Ogawa K, Suzuki F, Katagiri K, Saito D, Ikeda A, Horii A, Watanabe M, Igimi S.
タイトル Treatment with Lactobacillus Retards the Tumor Growth of Head and Neck Squamous Cell Carcinoma Cells Inoculated in Mice.
ジャーナル Tohoku J Exp Med
Abstract Bacteria have been used for more than a century to treat solid tumors. Because solid tumors generate an anaerobic environment, we evaluated the anti-tumor effect of the obligate anaerobe strain KK378, derived from Lactobacillus casei (L. casei), using mice bearing head and neck cancer. Wild-type L. casei is a nonpathogenic bacterium that is commonly used in foods. Moreover, patients with head and neck squamous cell carcinoma often have multiple cancers and cervical lymph node metastasis that can be directly sensed beneath the skin. To establish the animal model bearing head and neck cancer, we inoculated each of human squamous cell carcinoma cell lines, SAS, HSQ89, and HSC2, on the back skin of BALB/cSlc-nu/nu mice. After tumor formation, L. casei KK378 was administered directly into the tumor, and tumor size and serum cytokine levels were analyzed. Mice injected with 108 cfu of L. casei KK378 showed reduction in tumor growth compared with PBS control; especially, the SAS tumor was significantly reduced (p = 0.008). Administered L. casei KK378 was detected in tumor tissues but not in normal tissues (liver, kidney, and lung) of SAS tumor-bearing mice, which was associated with increased blood cytokines (TNF-α, IFN-γ, IL-5, IL-10, and IL-12). Among these cytokines, the serum levels of IFN-γ and TNF-α were significantly increased (p < 0.05). In conclusion, L. casei KK378 infection may suppress tumor growth by inducing the host immune response. Direct injection of Lactobacillus into the tumor could be a potential strategy to treat head and neck squamous cell carcinoma.
巻・号 245(4)
ページ 269-275
公開日 2018-8-1
DOI 10.1620/tjem.245.269
PMID 30158369
MeSH Animals Carcinoma, Squamous Cell / blood Carcinoma, Squamous Cell / pathology* Carcinoma, Squamous Cell / therapy* Cell Line, Tumor Cell Proliferation Cytokines / blood Head and Neck Neoplasms / blood Head and Neck Neoplasms / pathology* Head and Neck Neoplasms / therapy* Lacticaseibacillus casei / physiology* Mice, Inbred BALB C Squamous Cell Carcinoma of Head and Neck
IF 1.441
引用数 2
リソース情報
ヒト・動物細胞 HSC-2(RCB1945) HSQ-89(RCB0789)