論文 - 詳細
| RRC ID | 54139 |
|---|---|
| 著者 | Hasegawa Y, Hoshino Y, Ibrahim AE, Kato K, Daitoku Y, Tanimoto Y, Ikeda Y, Oishi H, Takahashi S, Yoshiki A, Yagami K, Iseki H, Mizuno S, Sugiyama F. |
| タイトル | Generation of CRISPR/Cas9-mediated bicistronic knock-in ins1-cre driver mice. |
| ジャーナル | Exp Anim |
| Abstract |
In the present study, we generated novel cre driver mice for gene manipulation in pancreatic β cells. Using the CRISPR/Cas9 system, stop codon sequences of Ins1 were targeted for insertion of cre, including 2A sequences. A founder of C57BL/6J-Ins1(em1 (cre) Utr) strain was produced from an oocyte injected with pX330 containing the sequences encoding gRNA and Cas9 and a DNA donor plasmid carrying 2A-cre. (R26GRR x C57BL/6J-Ins1(em1 (cre) Utr)) F1 mice were histologically characterized for cre-loxP recombination in the embryonic and adult stages; cre-loxP recombination was observed in all pancreatic islets examined in which almost all insulin-positive cells showed tdsRed fluorescence, suggesting β cell-specific recombination. Furthermore, there were no significant differences in results of glucose tolerance test among genotypes (homo/hetero/wild). Taken together, these observations indicated that C57BL/6J-Ins1(em1 (cre) Utr) is useful for studies of glucose metabolism and the strategy of bicistronic cre knock-in using the CRISPR/Cas9 system could be useful for production of cre driver mice. |
| 巻・号 | 65(3) |
| ページ | 319-27 |
| 公開日 | 2016-7-29 |
| DOI | 10.1538/expanim.16-0016 |
| PMID | 27053096 |
| PMC | PMC4976246 |
| MeSH | Animals CRISPR-Cas Systems* Codon, Terminator / genetics Crk-Associated Substrate Protein / administration & dosage Gene Editing / methods* Glucose / metabolism Injections Insulin / genetics* Insulin-Secreting Cells* Integrases / administration & dosage Integrases / genetics* Mice, Inbred C57BL Mice, Mutant Strains* / genetics Mutagenesis, Insertional Oocytes RNA / administration & dosage Recombination, Genetic |
| IF | 1.574 |
| 引用数 | 7 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| 遺伝子材料 | px330-Ins1 (RDB13945) pIns1/2A-cre (RDB13946) p2color-Ins1 (RDB13947) p2color (RDB13948) |