RRC ID 54159
Author Kawagoshi T, Shiomi N, Takahashi H, Watanabe Y, Fuma S, Doi K, Kawaguchi I, Aoki M, Kubota M, Furuhata Y, Shigemura Y, Mizoguchi M, Yamada F, Tomozawa M, Sakamoto SH, Yoshida S, Kubota Y.
Title Chromosomal Aberrations in Large Japanese Field Mice (Apodemus speciosus) Captured near Fukushima Dai-ichi Nuclear Power Plant.
Journal Environ Sci Technol
Abstract Since the Fukushima Dai-ichi Nuclear Power Plant accident, radiation effects on nonhuman biota in the contaminated areas have been a major concern. Here, we analyzed the frequencies of chromosomal aberrations (translocations and dicentrics) in the splenic lymphocytes of large Japanese field mice (Apodemus speciosus) inhabiting Fukushima Prefecture. A. speciosus chromosomes 1, 2, and 5 were flow-sorted in order to develop A. speciosus chromosome-specific painting probes, and FISH (fluorescence in situ hybridization) was performed using these painting probes to detect the translocations and dicentrics. The average frequency of the translocations and dicentrics per cell in the heavily contaminated area was significantly higher than the frequencies in the case of the noncontaminated control area and the slightly and moderately contaminated areas, and this aberration frequency in individual mice tended to roughly increase with the estimated dose rates and accumulated doses. In all four sampling areas, the proportion of aberrations occurring in chromosome 2 was approximately >3 times higher than that in chromosomes 1 and 5, which suggests that A. speciosus chromosome 2 harbors a fragile site that is highly sensitive to chromosome breaks induced by cellular stress such as DNA replication. The elevated frequency of chromosomal aberrations in A. speciosus potentially resulting from the presence of a fragile site in chromosome 2 might make it challenging to observe the mild effect of chronic low-dose-rate irradiation on the induction of chromosomal aberrations in A. speciosus inhabiting the contaminated areas of Fukushima.
Volume 51(8)
Pages 4632-4641
Published 2017-4-18
DOI 10.1021/acs.est.6b06210
PMID 28363014
MeSH Animals Chromosome Aberrations* Fukushima Nuclear Accident* In Situ Hybridization, Fluorescence Mice Murinae / genetics* Nuclear Power Plants*
IF 7.864
Times Cited 11
Resource
DNA material Painting probe DNA for A. speciosus chromosome 1 (RDB16699) chromosome 2 (RDB16700) chromosome 3 and 4 (RDB16701) chromosome 5 (RDB16702) chromosome 6 (RDB16703) chromosome 7 (RDB16704) chromosome 8 (RDB16705) X chromosome (RDB16706)