RRC ID 54162
Author Sharma A, Halder S, Felix M, Nisaa K, Deshpande G, Prasad M.
Title Insulin signaling modulates border cell movement in Drosophila oogenesis.
Journal Development
Abstract As collective cell migration is intimately involved in different aspects of metazoan development, molecular mechanisms underlying this process are being explored in a variety of developmental contexts. Border cell (BC) migration during Drosophila oogenesis has emerged as an excellent genetic model for studying collective cell migration. BCs are of epithelial origin but acquire partial mesenchymal characteristics before migrating as a group towards the oocyte. Here, we report that insulin signaling modulates collective BC movement during Drosophila oogenesis. Supporting the involvement of Insulin pathway, we demonstrate that compromising Insulin-like Receptor (InR) levels in BCs, inhibits their migration. Furthermore, we show that canonical Insulin signaling pathway components participate in this process. Interestingly, visualization of InR-depleted BC clusters, using time-lapse imaging, revealed a delay in detachment of BC clusters from the surrounding anterior follicle cells and altered protrusion dynamics. Lastly, based on genetic interactions between InR, the polarity determinant, par-1 and a regulatory subunit of Drosophila Myosin (spaghetti squash), we propose that Insulin signaling likely influences par-1 activity to engineer border cell detachment and subsequent movement via Drosophila Myosin.
Volume 145(14)
Published 2018-7-30
DOI 10.1242/dev.166165
PII dev.166165
PMID 29950391
PMC PMC6078333
MeSH Animals Cell Movement* Cell Surface Extensions / metabolism Drosophila Proteins / metabolism Drosophila melanogaster / cytology* Drosophila melanogaster / metabolism* Female Insulin / metabolism* Oogenesis* Receptor, Insulin / metabolism Signal Transduction*
IF 5.611
Times Cited 2
Drosophila 18402Ra-2