RRC ID 54166
Author Hoshikawa K, Fujita S, Renhu N, Ezura K, Yamamoto T, Nonaka S, Ezura H, Miura K.
Title Efficient transient protein expression in tomato cultivars and wild species using agroinfiltration-mediated high expression system.
Journal Plant Cell Rep
Abstract KEY MESSAGE:The new transient protein expression system using the pBYR2HS vector is applicable to several tomato cultivars and wild species with high level of protein expression. Innovation and improvement of effective tools for transient protein expression in plant cells is critical for the development of plant biotechnology. We have created the new transient protein expression system using the pBYR2HS vector that led to about 4 mg/g fresh weight of protein expression in Nicotiana benthamiana. In this study, we validated the adaptability of this transient protein expression system by agroinfiltration to leaves and fruits of several tomato cultivars and wild species. Although the GFP protein was transiently expressed in the leaves and fruits of all tomato cultivars and wild species, we observed species-specific differences in protein expression. In particular, GFP protein expression was higher in the leaves and fruits of Micro-Tom, Solanum pimpinellifolium (0043) and S. pimpinellifolium (0049-w1) than in those of cultivars and wild species. Furthermore, Agrobacterium with GABA transaminase enhanced transient expression in tomato fruits of Micro-Tom. Taken together with these results, our system is applicable to several tomato cultivars and species as well as a model tomato, even though characteristics are often different among tomato cultivars or species. Thus, the system is an effective, simple, and valuable tool to achieve rapid transgene expression to examine gene function in tomato plant cells.
Volume 38(1)
Pages 75-84
Published 2019-1-1
DOI 10.1007/s00299-018-2350-1
PII 10.1007/s00299-018-2350-1
PMID 30328507
MeSH 4-Aminobutyrate Transaminase / genetics 4-Aminobutyrate Transaminase / metabolism Agrobacterium / genetics Agrobacterium / metabolism Gene Expression Regulation, Plant Lycopersicon esculentum / genetics Lycopersicon esculentum / metabolism* Lycopersicon esculentum / microbiology Plant Proteins / genetics Plant Proteins / metabolism* Plants, Genetically Modified / genetics Plants, Genetically Modified / metabolism*
IF 3.825
Times Cited 6
Tomato TOMJPF00001 TOMJPF00002(Moneymaker) TOMJPF00003(Aichi-first) TOMJPF00004(Ailsa Craig) TOMJPF00005(M82) TOMJPF00006(Rutgers) TOMJPF00007(Ponderosa) TOMJPF00015(Ueleie 106 WP)