RRC ID |
54171
|
著者 |
Bin Saifullah MA, Nagai T, Kuroda K, Wulaer B, Nabeshima T, Kaibuchi K, Yamada K.
|
タイトル |
Cell type-specific activation of mitogen-activated protein kinase in D1 receptor-expressing neurons of the nucleus accumbens potentiates stimulus-reward learning in mice.
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ジャーナル |
Sci Rep
|
Abstract |
Medium spiny neurons (MSN) in the nucleus accumbens (NAc) are a fundamental component of various aspects of motivated behavior. Although mitogen-activated protein kinase (MAPK) signaling plays a crucial role in several types of learning, the cell type-specific role of MAPK pathway in stimulus-reward learning and motivation remains unclear. We herein investigated the role of MAPK in accumbal MSNs in reward-associated learning and memory. During the acquisition of Pavlovian conditioning, the number of phosphorylated MAPK1/3-positive cells was increased significantly and exclusively in the NAc core by 7-days of extensive training. MAPK signaling in the respective D1R- and D2R-MSNs was manipulated by transfecting an adeno-associated virus (AAV) plasmid into the NAc of Drd1a-Cre and Drd2-Cre transgenic mice. Potentiation of MAPK signaling shifted the learning curve of Pavlovian conditioning to the left only in Drd1a-Cre mice, whereas such manipulation in D2R-MSNs had negligible effects. In contrast, MAPK manipulation in D2R-MSNs of the NAc core significantly increased motivation for food rewards as found in Drd1a-Cre mice. These results suggest that MAPK signaling in the D1R-MSNs of NAc core plays an important role in stimulus-reward learning, while MAPK signaling in both D1R- and D2R-MSNs is involved in motivation for natural rewards.
|
巻・号 |
8(1)
|
ページ |
14413
|
公開日 |
2018-9-26
|
DOI |
10.1038/s41598-018-32840-1
|
PII |
10.1038/s41598-018-32840-1
|
PMID |
30258218
|
PMC |
PMC6158283
|
MeSH |
Animals
Conditioning, Classical
Dopaminergic Neurons / cytology
Dopaminergic Neurons / metabolism*
Learning*
MAP Kinase Signaling System*
Male
Mice, Inbred C57BL
Motivation
Nucleus Accumbens / cytology*
Nucleus Accumbens / physiology
Phosphorylation
Receptors, Dopamine D1 / metabolism*
Receptors, Dopamine D2 / metabolism
Reward
|
IF |
3.998
|
引用数 |
1
|
リソース情報 |
実験動物マウス |
RBRC02332
RBRC03111 |