| RRC ID |
54354
|
| Author |
Kobayashi S, Hara A, Isagawa T, Manabe I, Takeda K, MaruYama T.
|
| Title |
The nuclear IκB family protein IκBNS influences the susceptibility to experimental autoimmune encephalomyelitis in a murine model.
|
| Journal |
PLoS One
|
| Abstract |
The nuclear IκB family protein IκBNS is expressed in T cells and plays an important role in Interferon (IFN)-γ and Interleukin (IL)-2 production. IκB-ζ, the most similar homolog of IκBNS, plays an important role in the generation of T helper (Th)17 cells in cooperation with RORγt, a master regulator of Th17 cells. Thus, IκB-ζ deficient mice are resistant to Th17-dependent experimental autoimmune encephalomyelitis (EAE). However, IκB-ζ deficient mice develop the autoimmune-like Sjögren syndrome with aging. Here we found that IκBNS-deficient (Nfkbid-/-) mice show resistance against developing Th17-dependent EAE. We found that Nfkbid-/- T cells have decreased expression of IL-17-related genes and RORγt in response to Transforming Growth Factor (TGF)-β1 and IL-6 stimulation. Thus, IκBNS plays a pivotal role in the generation of Th17 cells and in the control of Th17-dependent EAE.
|
| Volume |
9(10)
|
| Pages |
e110838
|
| Published |
2014-1-1
|
| DOI |
10.1371/journal.pone.0110838
|
| PII |
PONE-D-14-24165
|
| PMID |
25347393
|
| PMC |
PMC4210207
|
| MeSH |
Animals
Cell Differentiation
Disease Models, Animal
Disease Susceptibility*
Encephalomyelitis, Autoimmune, Experimental / genetics
Encephalomyelitis, Autoimmune, Experimental / immunology
Encephalomyelitis, Autoimmune, Experimental / metabolism*
Gene Expression Regulation
I-kappa B Proteins / genetics
I-kappa B Proteins / metabolism*
Mice
Mice, Knockout
NF-kappa B / genetics
Nuclear Receptor Subfamily 1, Group F, Member 3 / genetics
Nuclear Receptor Subfamily 1, Group F, Member 3 / metabolism
T-Lymphocyte Subsets / cytology
T-Lymphocyte Subsets / immunology
T-Lymphocyte Subsets / metabolism
Th17 Cells / cytology
Th17 Cells / immunology
Th17 Cells / metabolism
|
| IF |
2.74
|
| Times Cited |
20
|
| Resource |
| DNA material |
pGL4.12-mouse IL-17A promoter (-6647 to +1) (RDB16724)
pcDNA3-FLAG-mouse ROR gamma t(RDB16728)
pEF6-FLAG-mouse IkappaBNS (RDB16725)
pEF6-HA-mouse IkappaBNS (RDB16726)
pEF6-Myc-mouse IkappaBNS (RDB16727) |
