| RRC ID |
54373
|
| 著者 |
Sandler JE, Irizarry J, Stepanik V, Dunipace L, Amrhein H, Stathopoulos A.
|
| タイトル |
A Developmental Program Truncates Long Transcripts to Temporally Regulate Cell Signaling.
|
| ジャーナル |
Dev Cell
|
| Abstract |
Rapid mitotic divisions and a fixed transcription rate limit the maximal length of transcripts in early Drosophila embryos. Previous studies suggested that transcription of long genes is initiated but aborted, as early nuclear divisions have short interphases. Here, we identify long genes that are expressed during short nuclear cycles as truncated transcripts. The RNA binding protein Sex-lethal physically associates with transcripts for these genes and is required to support early termination to specify shorter transcript isoforms in early embryos of both sexes. In addition, one truncated transcript for the gene short-gastrulation encodes a product in embryos that functionally relates to a previously characterized dominant-negative form, which maintains TGF-β signaling in the off-state. In summary, our results reveal a developmental program of short transcripts functioning to help temporally regulate Drosophila embryonic development, keeping cell signaling at early stages to a minimum in order to support its proper initiation at cellularization.
|
| 巻・号 |
47(6)
|
| ページ |
773-784.e6
|
| 公開日 |
2018-12-17
|
| DOI |
10.1016/j.devcel.2018.11.019
|
| PII |
S1534-5807(18)30975-4
|
| PMID |
30562515
|
| PMC |
PMC6506262
|
| MeSH |
Animals
Cell Nucleus / metabolism
Drosophila Proteins / metabolism
Drosophila Proteins / physiology
Drosophila melanogaster / genetics
Drosophila melanogaster / metabolism
Embryo, Nonmammalian / metabolism
Embryonic Development / physiology
Gene Expression Profiling / methods
Gene Expression Regulation, Developmental / physiology*
Mitosis / physiology
Morphogenesis
RNA Isoforms / physiology
RNA-Binding Proteins / physiology
Regulatory Elements, Transcriptional / physiology
Signal Transduction / genetics*
Terminator Regions, Genetic / physiology
Transcription, Genetic / physiology*
|
| IF |
10.092
|
| 引用数 |
4
|
| リソース情報 |
| ショウジョウバエ |
BX-0003
Cas-0004 |
