論文 - 詳細
| RRC ID | 54389 |
|---|---|
| 著者 | Igarashi H, Koizumi K, Kaneko R, Ikeda K, Egawa R, Yanagawa Y, Muramatsu S, Onimaru H, Ishizuka T, Yawo H. |
| タイトル | A Novel Reporter Rat Strain That Conditionally Expresses the Bright Red Fluorescent Protein tdTomato. |
| ジャーナル | PLoS One |
| Abstract |
Despite the strength of the Cre/loxP recombination system in animal models, its application in rats trails that in mice because of the lack of relevant reporter strains. Here, we generated a floxed STOP tdTomato rat that conditionally expresses a red fluorescent protein variant (tdTomato) in the presence of exogenous Cre recombinase. The tdTomato signal vividly visualizes neurons including their projection fibers and spines without any histological enhancement. In addition, a transgenic rat line (FLAME) that ubiquitously expresses tdTomato was successfully established by injecting intracytoplasmic Cre mRNA into fertilized ova. Our rat reporter system will facilitate connectome studies as well as the visualization of the fine structures of genetically identified cells for long periods both in vivo and ex vivo. Furthermore, FLAME is an ideal model for organ transplantation research owing to improved traceability of cells/tissues. |
| 巻・号 | 11(5) |
| ページ | e0155687 |
| 公開日 | 2016-1-1 |
| DOI | 10.1371/journal.pone.0155687 |
| PII | PONE-D-16-00760 |
| PMID | 27195805 |
| PMC | PMC4873025 |
| MeSH | Animals Cell Lineage Chromosomes, Artificial, Bacterial Dependovirus Electroporation Erythrocytes / cytology Female Fertilization Fibroblasts / metabolism Genes, Reporter Hippocampus / metabolism Imaging, Three-Dimensional Integrases Luminescent Proteins / genetics* Macrophages / metabolism Male RNA, Messenger / metabolism Rats Rats, Transgenic* Transgenes |
| IF | 2.74 |
| 引用数 | 8 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pAcGFP-NCre (RDB16797) |