論文 - 詳細
| RRC ID | 54393 |
|---|---|
| 著者 | Hososhima S, Yuasa H, Ishizuka T, Hoque MR, Yamashita T, Yamanaka A, Sugano E, Tomita H, Yawo H. |
| タイトル | Near-infrared (NIR) up-conversion optogenetics. |
| ジャーナル | Sci Rep |
| Abstract |
Non-invasive remote control technologies designed to manipulate neural functions have been long-awaited for the comprehensive and quantitative understanding of neuronal network in the brain as well as for the therapy of neurological disorders. Recently, it has become possible for the neuronal activity to be optically manipulated using biological photo-reactive molecules such as channelrhodopsin (ChR)-2. However, ChR2 and its relatives are mostly reactive to visible light, which does not effectively penetrate through biological tissues. In contrast, near-infrared (NIR) light (650-1450 nm) penetrates deep into the tissues because biological systems are almost transparent to light within this so-called 'imaging window'. Here we used lanthanide nanoparticles (LNPs), composed of rare-earth elements, as luminous bodies to activate ChRs since they absorb low-energy NIR light to emit high-energy visible light (up-conversion). Here, we created a new type of optogenetic system which consists of the donor LNPs and the acceptor ChRs. The NIR laser irradiation emitted visible light from LNPs, then induced the photo-reactive responses in the near-by cells that expressed ChRs. However, there remains room for large improvements in the energy efficiency of the LNP-ChR system. |
| 巻・号 | 5 |
| ページ | 16533 |
| 公開日 | 2015-11-10 |
| DOI | 10.1038/srep16533 |
| PII | srep16533 |
| PMID | 26552717 |
| PMC | PMC4639720 |
| MeSH | Animals Infrared Rays* Lanthanoid Series Elements Metal Nanoparticles Mice Neurons / physiology Optogenetics* Rats Spectroscopy, Near-Infrared |
| IF | 3.998 |
| 引用数 | 44 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 11 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| 遺伝子材料 | pCMV-C1V1-Venus (RDB16793) pCMV-PsChR-Venus (RDB16794) pCaMKIIa-C1V1-Venus (RDB16795) pCaMKIIa-PsChR-Venus (RDB16796) |