RRC ID 54401
Author Valanne S, Salminen TS, Järvelä-Stölting M, Vesala L, Rämet M.
Title Immune-inducible non-coding RNA molecule lincRNA-IBIN connects immunity and metabolism in Drosophila melanogaster.
Journal PLoS Pathog
Abstract Non-coding RNAs have important roles in regulating physiology, including immunity. Here, we performed transcriptome profiling of immune-responsive genes in Drosophila melanogaster during a Gram-positive bacterial infection, concentrating on long non-coding RNA (lncRNA) genes. The gene most highly induced by a Micrococcus luteus infection was CR44404, named Induced by Infection (lincRNA-IBIN). lincRNA-IBIN is induced by both Gram-positive and Gram-negative bacteria in Drosophila adults and parasitoid wasp Leptopilina boulardi in Drosophila larvae, as well as by the activation of the Toll or the Imd pathway in unchallenged flies. We show that upon infection, lincRNA-IBIN is expressed in the fat body, in hemocytes and in the gut, and its expression is regulated by NF-κB signaling and the chromatin modeling brahma complex. In the fat body, overexpression of lincRNA-IBIN affected the expression of Toll pathway -mediated genes. Notably, overexpression of lincRNA-IBIN in unchallenged flies elevated sugar levels in the hemolymph by enhancing the expression of genes important for glucose retrieval. These data show that lncRNA genes play a role in Drosophila immunity and indicate that lincRNA-IBIN acts as a link between innate immune responses and metabolism.
Volume 15(1)
Pages e1007504
Published 2019-1-1
DOI 10.1371/journal.ppat.1007504
PMID 30633769
PMC PMC6345493
MeSH Animals Chromatin Assembly and Disassembly Drosophila Proteins / genetics Drosophila melanogaster / genetics Drosophila melanogaster / immunology Drosophila melanogaster / microbiology Gram-Negative Bacteria / immunology Gram-Positive Bacteria / immunology Gram-Positive Bacterial Infections / genetics* Gram-Positive Bacterial Infections / immunology Hemolymph / metabolism Immunity, Innate / genetics NF-kappa B / metabolism RNA, Long Noncoding / genetics* RNA, Long Noncoding / immunology* Signal Transduction Transcriptome / genetics Wasps / genetics Wasps / immunology
IF 6.218
Times Cited 9
Drosophila 5848R-3