Reference - Detail
| RRC ID | 54408 |
|---|---|
| Author | Kunii M, Kitahama Y, Fukushima EO, Seki H, Muranaka T, Yoshida Y, Aoyama Y. |
| Title | β-Amyrin oxidation by oat CYP51H10 expressed heterologously in yeast cells: the first example of CYP51-dependent metabolism other than the 14-demethylation of sterol precursors. |
| Journal | Biol Pharm Bull |
| Abstract |
CYP51 has been recognized as a unique CYP family that consists of one isolated molecular species, a sterol 14-demethylase essential for sterol biosynthesis. However, another CYP51 gene classified as the CYP51H subfamily has been identified in higher plants, in addition to a sterol 14-demethylase gene, CYP51G1. To shed light on the function of this "second CYP51", oat CYP51H10 was introduced into the β-amyrin-producing yeast cells, and the effect of the expressed CYP51H10 on β-amyrin metabolism in the host cells was examined. In the CYP51H10-introduced cells, β-amyrin was converted to a metabolite with 12,13-epoxy and one additional hydroxyl group. Since the 12,13-epoxy group introduced into β-amyrin ring is an essential structure of avenacin A-1, a triterpene glycoside produced in oat from β-amyrin, the present findings indicate the contribution of CYP51H10 to avenacin A-1 biosynthesis from β-amyrin. This is the first study showing a second function of the CYP51 family. |
| Volume | 35(5) |
| Pages | 801-4 |
| Published | 2012-1-1 |
| DOI | 10.1248/bpb.35.801 |
| PII | JST.JSTAGE/bpb/35.801 |
| PMID | 22687421 |
| MeSH | Avena / enzymology Avena / genetics* Genes, Plant* Oleanolic Acid / analogs & derivatives* Oleanolic Acid / metabolism Phytosterols / biosynthesis Saponins / biosynthesis* Sterol 14-Demethylase / genetics* Sterol 14-Demethylase / metabolism Yeasts / genetics Yeasts / metabolism |
| IF | 1.863 |
| Times Cited | 18 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| DNA material | Oat CYP51H10/pBKS- (RDB16759) |