| RRC ID |
54427
|
| Author |
Shimatani Z, Ariizumi T, Fujikura U, Kondo A, Ezura H, Nishida K.
|
| Title |
Targeted Base Editing with CRISPR-Deaminase in Tomato.
|
| Journal |
Methods Mol Biol
|
| Abstract |
The Target-AID system, consisting of a complex of cytidine deaminase and deficient CRISPR/Cas9, enables highly specific genomic nucleotide substitutions without the need for template DNA. The Cas9-fused cytidine deaminase is guided by sgRNAs and catalyzes the conversion of cytosine to uracil. The resulting U-G DNA mismatches trigger nucleotide substitutions (C to T or G to A) through DNA replication and repair pathways. Target-AID also retains the benefits of conventional CRISPR/Cas9 including robustness in various organisms, high targeting efficiency, and multiplex simultaneous gene editing. Our research group recently developed plant-optimized Target-AID system and demonstrated targeted base editing in tomato and rice. In this chapter, we introduce methods for Target-AID application in tomato.
|
| Volume |
1917
|
| Pages |
297-307
|
| Published |
2019-1-1
|
| DOI |
10.1007/978-1-4939-8991-1_22
|
| PMID |
30610645
|
| MeSH |
CRISPR-Cas Systems / genetics*
Clustered Regularly Interspaced Short Palindromic Repeats / genetics*
Cytidine Deaminase / genetics
Gene Editing / methods*
Solanum lycopersicum / genetics*
|
| Resource |
| Tomato |
TOMJPF00001 |
