RRC ID 54427
著者 Shimatani Z, Ariizumi T, Fujikura U, Kondo A, Ezura H, Nishida K.
タイトル Targeted Base Editing with CRISPR-Deaminase in Tomato.
ジャーナル Methods Mol Biol
Abstract The Target-AID system, consisting of a complex of cytidine deaminase and deficient CRISPR/Cas9, enables highly specific genomic nucleotide substitutions without the need for template DNA. The Cas9-fused cytidine deaminase is guided by sgRNAs and catalyzes the conversion of cytosine to uracil. The resulting U-G DNA mismatches trigger nucleotide substitutions (C to T or G to A) through DNA replication and repair pathways. Target-AID also retains the benefits of conventional CRISPR/Cas9 including robustness in various organisms, high targeting efficiency, and multiplex simultaneous gene editing. Our research group recently developed plant-optimized Target-AID system and demonstrated targeted base editing in tomato and rice. In this chapter, we introduce methods for Target-AID application in tomato.
巻・号 1917
ページ 297-307
公開日 2019-1-1
DOI 10.1007/978-1-4939-8991-1_22
PMID 30610645
MeSH CRISPR-Cas Systems / genetics* Clustered Regularly Interspaced Short Palindromic Repeats / genetics* Cytidine Deaminase / genetics Gene Editing / methods* Solanum lycopersicum / genetics*
リソース情報
トマト TOMJPF00001