論文 - 詳細
| RRC ID | 54427 |
|---|---|
| 著者 | Shimatani Z, Ariizumi T, Fujikura U, Kondo A, Ezura H, Nishida K. |
| タイトル | Targeted Base Editing with CRISPR-Deaminase in Tomato. |
| ジャーナル | Methods Mol Biol |
| Abstract |
The Target-AID system, consisting of a complex of cytidine deaminase and deficient CRISPR/Cas9, enables highly specific genomic nucleotide substitutions without the need for template DNA. The Cas9-fused cytidine deaminase is guided by sgRNAs and catalyzes the conversion of cytosine to uracil. The resulting U-G DNA mismatches trigger nucleotide substitutions (C to T or G to A) through DNA replication and repair pathways. Target-AID also retains the benefits of conventional CRISPR/Cas9 including robustness in various organisms, high targeting efficiency, and multiplex simultaneous gene editing. Our research group recently developed plant-optimized Target-AID system and demonstrated targeted base editing in tomato and rice. In this chapter, we introduce methods for Target-AID application in tomato. |
| 巻・号 | 1917 |
| ページ | 297-307 |
| 公開日 | 2019-1-1 |
| DOI | 10.1007/978-1-4939-8991-1_22 |
| PMID | 30610645 |
| MeSH | CRISPR-Cas Systems / genetics* Clustered Regularly Interspaced Short Palindromic Repeats / genetics* Cytidine Deaminase / genetics Gene Editing / methods* Solanum lycopersicum / genetics* |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| トマト | TOMJPF00001 |