論文 - 詳細
| RRC ID | 54434 |
|---|---|
| 著者 | Xu C, Tang HW, Hung RJ, Hu Y, Ni X, Housden BE, Perrimon N. |
| タイトル | The Septate Junction Protein Tsp2A Restricts Intestinal Stem Cell Activity via Endocytic Regulation of aPKC and Hippo Signaling. |
| ジャーナル | Cell Rep |
| Abstract |
Hippo signaling and the activity of its transcriptional coactivator, Yorkie (Yki), are conserved and crucial regulators of tissue homeostasis. In the Drosophila midgut, after tissue damage, Yki activity increases to stimulate stem cell proliferation, but how Yki activity is turned off once the tissue is repaired is unknown. From an RNAi screen, we identified the septate junction (SJ) protein tetraspanin 2A (Tsp2A) as a tumor suppressor. Tsp2A undergoes internalization to facilitate the endocytic degradation of atypical protein kinase C (aPKC), a negative regulator of Hippo signaling. In the Drosophila midgut epithelium, adherens junctions (AJs) and SJs are prominent in intestinal stem cells or enteroblasts (ISCs or EBs) and enterocytes (ECs), respectively. We show that when ISCs differentiate toward ECs, Tsp2A is produced, participates in SJ assembly, and turns off aPKC and Yki-JAK-Stat activity. Altogether, our study uncovers a mechanism allowing the midgut to restore Hippo signaling and restrict proliferation once tissue repair is accomplished. |
| 巻・号 | 26(3) |
| ページ | 670-688.e6 |
| 公開日 | 2019-1-15 |
| DOI | 10.1016/j.celrep.2018.12.079 |
| PII | S2211-1247(18)32024-2 |
| PMID | 30650359 |
| PMC | PMC6394833 |
| MeSH | Cell Proliferation Humans Intestines / physiopathology* Protein Kinase C / metabolism* Signal Transduction Stem Cells / metabolism* Tetraspanins / metabolism* |
| IF | 7.815 |
| 引用数 | 9 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 17 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | 11415R-2 |