RRC ID 54443
著者 Iwakami S, Kamidate Y, Yamaguchi T, Ishizaka M, Endo M, Suda H, Nagai K, Sunohara Y, Toki S, Uchino A, Tominaga T, Matsumoto H.
タイトル CYP81A P450s are involved in concomitant cross-resistance to acetolactate synthase and acetyl-CoA carboxylase herbicides in Echinochloa phyllopogon.
ジャーナル New Phytol
Abstract Californian populations of Echinochloa phyllopogon have evolved multiple-herbicide resistance (MHR), posing a threat to rice production in California. Previously, we identified two CYP81A cytochrome P450 genes whose overexpression is associated with resistance to acetolactate synthase (ALS) inhibitors from two chemical groups. Resistance mechanisms to other herbicides remain unknown. We analyzed the sensitivity of an MHR line to acetyl-CoA carboxylase (ACCase) inhibitors from three chemical groups, followed by an analysis of herbicide metabolism and segregation of resistance of the progenies in sensitive (S) and MHR lines. ACCase herbicide metabolizing function was investigated in the two previously identified P450s. MHR plants exhibited resistance to all the ACCase inhibitors by enhanced herbicide metabolism. Resistance to the ACCase inhibitors segregated in a 3 : 1 ratio in the F2 generation and completely co-segregated with ALS inhibitor resistance in F6 lines. Expression of the respective P450 genes conferred resistance to the three herbicides in rice, which is in line with the detection of hydroxylated herbicide metabolites in vivo in transformed yeast. CYP81As are super P450s that metabolize multiple herbicides from five chemical classes, and concurrent overexpression of the P450s induces metabolism-based resistance to the three ACCase inhibitors in MHR E. phyllopogon, as it does to ALS inhibitors.
巻・号 221(4)
ページ 2112-2122
公開日 2019-3-1
DOI 10.1111/nph.15552
PMID 30347444
MeSH Acetolactate Synthase / metabolism* Acetyl-CoA Carboxylase / metabolism* Crosses, Genetic Cytochrome P-450 Enzyme System / metabolism Echinochloa / drug effects Echinochloa / enzymology* Echinochloa / genetics Enzyme Inhibitors / pharmacology Herbicide Resistance* Herbicides / toxicity* Hordeum / genetics Saccharomyces cerevisiae / metabolism
IF 7.299
引用数 10
リソース情報
オオムギ Golden Promise