RRC ID 54446
Author Toju H, Okayasu K, Notaguchi M.
Title Leaf-associated microbiomes of grafted tomato plants.
Journal Sci Rep
Abstract Bacteria and fungi form complex communities (microbiomes) in above- and below-ground organs of plants, contributing to hosts' growth and survival in various ways. Recent studies have suggested that host plant genotypes control, at least partly, plant-associated microbiome compositions. However, we still have limited knowledge of how microbiome structures are determined in/on grafted crop plants, whose above-ground (scion) and below-ground (rootstock) genotypes are different with each other. By using eight varieties of grafted tomato plants, we examined how rootstock genotypes could determine the assembly of leaf endophytic microbes in field conditions. An Illumina sequencing analysis showed that both bacterial and fungal community structures did not significantly differ among tomato plants with different rootstock genotypes: rather, sampling positions in the farmland contributed to microbiome variation in a major way. Nonetheless, a further analysis targeting respective microbial taxa suggested that some bacteria and fungi could be preferentially associated with particular rootstock treatments. Specifically, a bacterium in the genus Deinococcus was found disproportionately from ungrafted tomato individuals. In addition, yeasts in the genus Hannaella occurred frequently on the tomato individuals whose rootstock genotype was "Ganbarune". Overall, this study suggests to what extent leaf microbiome structures can be affected/unaffected by rootstock genotypes in grafted crop plants.
Volume 9(1)
Pages 1787
Published 2019-2-11
DOI 10.1038/s41598-018-38344-2
PII 10.1038/s41598-018-38344-2
PMID 30741982
PMC PMC6370777
MeSH Bacteria / genetics Bacteria / isolation & purification DNA, Plant / genetics DNA, Plant / isolation & purification Fungi / genetics Fungi / isolation & purification Microbiota* Plant Leaves / microbiology* RNA, Ribosomal, 16S / genetics Solanum lycopersicum / genetics Solanum lycopersicum / microbiology*
IF 4.011
Times Cited 5
Tomato TOMJPF00005(M82)