論文 - 詳細
| RRC ID | 54450 |
|---|---|
| 著者 | Dudin O, Merlini L, Martin SG. |
| タイトル | Spatial focalization of pheromone/MAPK signaling triggers commitment to cell-cell fusion. |
| ジャーナル | Genes Dev |
| Abstract |
Cell fusion is universal in eukaryotes for fertilization and development, but what signals this process is unknown. Here, we show in Schizosaccharomyces pombe that fusion does not require a dedicated signal but is triggered by spatial focalization of the same pheromone-GPCR (G-protein-coupled receptor)-MAPK signaling cascade that drives earlier mating events. Autocrine cells expressing the receptor for their own pheromone trigger fusion attempts independently of cell-cell contact by concentrating pheromone release at the fusion focus, a dynamic actin aster underlying the secretion of cell wall hydrolases. Pheromone receptor and MAPK cascade are similarly enriched at the fusion focus, concomitant with fusion commitment in wild-type mating pairs. This focalization promotes cell fusion by immobilizing the fusion focus, thus driving local cell wall dissolution. We propose that fusion commitment is imposed by a local increase in MAPK concentration at the fusion focus, driven by a positive feedback between fusion focus formation and focalization of pheromone release and perception. |
| 巻・号 | 30(19) |
| ページ | 2226-2239 |
| 公開日 | 2016-10-1 |
| DOI | 10.1101/gad.286922.116 |
| PII | 30/19/2226 |
| PMID | 27798845 |
| PMC | PMC5088570 |
| MeSH | Autocrine Communication / physiology MAP Kinase Signaling System / physiology* Pheromones / metabolism* Receptors, Pheromone / genetics Receptors, Pheromone / metabolism Schizosaccharomyces / cytology* Schizosaccharomyces / physiology* Schizosaccharomyces pombe Proteins / metabolism* |
| IF | 8.99 |
| 引用数 | 15 |
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オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
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