| RRC ID |
54453
|
| 著者 |
Kobayashi A, Autsavapromporn N, Ahmad TAFT, Oikawa M, Homma-Takeda S, Furusawa Y, Wang J, Konishi T.
|
| タイトル |
BYSTANDER WI-38 CELLS MODULATE DNA DOUBLE-STRAND BREAK REPAIR IN MICROBEAM-TARGETED A549 CELLS THROUGH GAP JUNCTION INTERCELLULAR COMMUNICATION.
|
| ジャーナル |
Radiat Prot Dosimetry
|
| Abstract |
Bi-directional signaling involved in radiation-induced bystander effect (RIBE) between irradiated carcinoma cells and their surrounding non-irradiated normal cells is relevant to radiation cancer therapy. Using the SPICE-NIRS microbeam, we delivered 500 protons to A549-GFP lung carcinoma cells, stably expressing H2B-GFP, which were co-cultured with normal WI-38 cells. The level of γ-H2AX, a marker for DNA double-strand breaks (DSB), was subsequently measured up to 24-h post-irradiation in both targeted and bystander cells. As a result, inhibition of gap junction intercellular communication (GJIC) attenuated DSB repair in targeted A549-GFP cells, and suppressed RIBE in bystander WI-38 cells but not in distant A549-GFP cells. This suggests that GJIC plays a two-way role through propagating DNA damage effect between carcinoma to normal cells and reversing the bystander signaling, also called 'rescue effect' from bystander cells to irradiated cells, to enhance the DSB repair in targeted cells.
|
| 巻・号 |
183(1-2)
|
| ページ |
142-146
|
| 公開日 |
2019-5-1
|
| DOI |
10.1093/rpd/ncy249
|
| PII |
5238185
|
| PMID |
30535060
|
| MeSH |
A549 Cells / radiation effects*
Bystander Effect / radiation effects
Cell Communication / radiation effects*
Cells, Cultured / radiation effects
Coculture Techniques
DNA Breaks, Double-Stranded / radiation effects*
DNA Repair
Fibroblasts / radiation effects
Gap Junctions / radiation effects*
Histones / analysis
Humans
Lung Neoplasms / radiotherapy*
Protons
Tumor Cells, Cultured / radiation effects*
|
| IF |
0.831
|
| 引用数 |
2
|
| リソース情報 |
| ヒト・動物細胞 |
A549(RCB0098)
WI-38(RCB0702) |
