RRC ID 54486
著者 Ito H, Sugawara T, Shinkai S, Mizukawa S, Kondo A, Senda H, Sawai K, Ito K, Suzuki S, Takaine M, Yoshida S, Imamura H, Kitamura K, Namba T, Tate SI, Ueno M.
タイトル Spindle pole body movement is affected by glucose and ammonium chloride in fission yeast.
ジャーナル Biochem Biophys Res Commun
Abstract The complexity of chromatin dynamics is orchestrated by several active processes. In fission yeast, the centromeres are clustered around the spindle pole body (SPB) and oscillate in a microtubule- and adenosine triphosphate (ATP)-dependent manner. However, whether and how SPB oscillation are affected by different environmental conditions remain poorly understood. In this study, we quantitated movements of the SPB component, which colocalizes with the centromere in fission yeast. We found that SPB movement was significantly reduced at low glucose concentrations. Movement of the SPB was also affected by the presence of ammonium chloride. Power spectral analysis revealed that periodic movement of the SPB is disrupted by low glucose concentrations. Measurement of ATP levels in living cells by quantitative single-cell imaging suggests that ATP levels are not the only determinant of SPB movement. Our results provide novel insight into how SPB movement is regulated by cellular energy status and additional factors such as the medium nutritional composition.
巻・号 511(4)
ページ 820-825
公開日 2019-4-16
DOI 10.1016/j.bbrc.2019.02.128
PII S0006-291X(19)30329-8
PMID 30846209
MeSH Adenosine Triphosphate / metabolism Ammonium Chloride / metabolism* Centromere / metabolism Glucose / metabolism* Schizosaccharomyces / cytology Schizosaccharomyces / metabolism* Spindle Pole Bodies / metabolism*
IF 2.705
引用数 2
リソース情報
酵母