| RRC ID |
54502
|
| 著者 |
Polburee P, Ohashi T, Tsai YY, Sumyai T, Lertwattanasakul N, Limtong S, Fujiyama K.
|
| タイトル |
Molecular cloning and overexpression of DGA1, an acyl-CoA-dependent diacylglycerol acyltransferase, in the oleaginous yeast Rhodosporidiobolus fluvialis DMKU-RK253.
|
| ジャーナル |
Microbiology (Reading)
|
| Abstract |
Triacylglycerol (TAG) is a major component of lipid storage in yeast. The acyl CoA: diacylgycerol acyltransferase (DGAT) that catalyzes the final and rate-limiting step in the production of TAG is rather interesting. Consequently, cloning and analysis of the gene-encoding TAG synthase, diacylglycerol acyltransferase gene (DGA1), of the oleaginous yeast Rhodosporidiobolus fluvialis DMKU-RK253 were undertaken. Analysis of the deduced amino acid sequence of DGA1 from R. fluvialis DMKU-RK253 (RfDGA1) showed similarity with the acyl-CoA:diacylglycerol acyltransferase 2 (DGAT2) from other organisms. The cDNA of RfDGA1 was cloned into the yeast expression vector pYES2 and heterologously overexpressed in Saccharomyces cerevisiae. One of the transformants showed a 1.6-fold increase in lipid content compared with the wild-type strain harbouring the pYES2 empty vector. Furthermore, DGA1 overexpression in R. fluvialis DMKU-RK253 resulted in a 2.5-fold increase in lipid content when compared with the wild-type strain, and no significant differences in fatty acid composition were observed between RfDGA1-overexpressed and wild-type strains. Taken together, our results supported our hypothesis that the RfDGA1 is a genetic factor that can be used for the development of a strain with improved lipid accumulation capabilities.
|
| 巻・号 |
164(1)
|
| ページ |
1-10
|
| 公開日 |
2018-1-1
|
| DOI |
10.1099/mic.0.000584
|
| PMID |
29182511
|
| MeSH |
Amino Acid Sequence
Basidiomycota / classification
Basidiomycota / enzymology*
Basidiomycota / genetics
Basidiomycota / metabolism
Biomass
Cloning, Molecular
Diacylglycerol O-Acyltransferase / genetics*
Diacylglycerol O-Acyltransferase / metabolism
Fatty Acids / analysis
Fungal Proteins / genetics*
Fungal Proteins / metabolism
Gene Expression*
Glycerol / metabolism
Lipid Metabolism / genetics*
Metabolic Engineering
Phylogeny
Saccharomyces cerevisiae / enzymology
Saccharomyces cerevisiae / genetics
Sequence Homology, Amino Acid
Triglycerides / biosynthesis
|
| IF |
1.922
|
| 引用数 |
3
|
| リソース情報 |
| 酵母 |
BY23849 |
