論文 - 詳細
| RRC ID | 54502 |
|---|---|
| 著者 | Polburee P, Ohashi T, Tsai YY, Sumyai T, Lertwattanasakul N, Limtong S, Fujiyama K. |
| タイトル | Molecular cloning and overexpression of DGA1, an acyl-CoA-dependent diacylglycerol acyltransferase, in the oleaginous yeast Rhodosporidiobolus fluvialis DMKU-RK253. |
| ジャーナル | Microbiology (Reading) |
| Abstract |
Triacylglycerol (TAG) is a major component of lipid storage in yeast. The acyl CoA: diacylgycerol acyltransferase (DGAT) that catalyzes the final and rate-limiting step in the production of TAG is rather interesting. Consequently, cloning and analysis of the gene-encoding TAG synthase, diacylglycerol acyltransferase gene (DGA1), of the oleaginous yeast Rhodosporidiobolus fluvialis DMKU-RK253 were undertaken. Analysis of the deduced amino acid sequence of DGA1 from R. fluvialis DMKU-RK253 (RfDGA1) showed similarity with the acyl-CoA:diacylglycerol acyltransferase 2 (DGAT2) from other organisms. The cDNA of RfDGA1 was cloned into the yeast expression vector pYES2 and heterologously overexpressed in Saccharomyces cerevisiae. One of the transformants showed a 1.6-fold increase in lipid content compared with the wild-type strain harbouring the pYES2 empty vector. Furthermore, DGA1 overexpression in R. fluvialis DMKU-RK253 resulted in a 2.5-fold increase in lipid content when compared with the wild-type strain, and no significant differences in fatty acid composition were observed between RfDGA1-overexpressed and wild-type strains. Taken together, our results supported our hypothesis that the RfDGA1 is a genetic factor that can be used for the development of a strain with improved lipid accumulation capabilities. |
| 巻・号 | 164(1) |
| ページ | 1-10 |
| 公開日 | 2018-1-1 |
| DOI | 10.1099/mic.0.000584 |
| PMID | 29182511 |
| MeSH | Amino Acid Sequence Basidiomycota / classification Basidiomycota / enzymology* Basidiomycota / genetics Basidiomycota / metabolism Biomass Cloning, Molecular Diacylglycerol O-Acyltransferase / genetics* Diacylglycerol O-Acyltransferase / metabolism Fatty Acids / analysis Fungal Proteins / genetics* Fungal Proteins / metabolism Gene Expression* Glycerol / metabolism Lipid Metabolism / genetics* Metabolic Engineering Phylogeny Saccharomyces cerevisiae / enzymology Saccharomyces cerevisiae / genetics Sequence Homology, Amino Acid Triglycerides / biosynthesis |
| IF | 1.922 |
| 引用数 | 3 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 酵母 | BY23849 |