RRC ID 54506
著者 Hartwick EW, Costantino DA, MacFadden A, Nix JC, Tian S, Das R, Kieft JS.
タイトル Ribosome-induced RNA conformational changes in a viral 3'-UTR sense and regulate translation levels.
ジャーナル Nat Commun
Abstract Structured RNA elements, programmed RNA conformational changes, and interactions between different RNA domains underlie many modes of regulating gene expression, mandating studies to understand the foundational principles that govern these phenomena. Exploring the structured 3' untranslated region (UTR) of a viral RNA, we discovered that different contexts of the 3'-UTR confer different abilities to enhance translation of an associated open reading frame. In one context, ribosome-induced conformational changes in a 'sensor' RNA domain affect a separate RNA 'functional' domain, altering translation efficiency. The structure of the entire 3'-UTR reveals that structurally distinct domains use a spine of continuously stacked bases and a strut-like linker to create a conduit for communication within the higher-order architecture. Thus, this 3'-UTR RNA illustrates how RNA can use programmed conformational changes to sense the translation status of an upstream open reading frame, then create a tuned functional response by communicating that information to other RNA elements.
巻・号 9(1)
ページ 5074
公開日 2018-11-29
DOI 10.1038/s41467-018-07542-x
PII 10.1038/s41467-018-07542-x
PMID 30498211
PMC PMC6265322
MeSH 3' Untranslated Regions / genetics* 5' Untranslated Regions / genetics Nucleic Acid Conformation Open Reading Frames / genetics Protein Biosynthesis RNA, Ribosomal / genetics* RNA, Viral / genetics
IF 11.878
引用数 1
リソース情報
遺伝子材料 Thermus thermophilus expression plasmid TEx18A06 (THR007206)