論文 - 詳細
| RRC ID | 54511 |
|---|---|
| 著者 | Fujihara M, Kaneko T, Inoue-Murayama M. |
| タイトル | Vitrification of canine ovarian tissues with polyvinylpyrrolidone preserves the survival and developmental capacity of primordial follicles. |
| ジャーナル | Sci Rep |
| Abstract |
Ovarian tissue cryopreservation combined with immature follicle development can preserve female fertility in wildlife, regardless of age or reproductive timing. To investigate the effects of different cryopreservation methods and cryoprotectants on follicular survival and developmental capacity, ovarian cortical pieces from 15 dogs were cryopreserved by slow freezing or vitrification with different additional cryoprotectants as follows: dimethyl sulfoxide (DMSO), polyvinylpyrrolidone (PVP), combined DMSO and PVP (each at half the concentration of when used independently), or none (control). Cryopreserved ovarian tissues were evaluated by neutral red staining, histology, and xenotransplantation assays. Among cryopreservation conditions tested, vitrification with combined DMSO and PVP significantly improved the maintenance of follicular morphology compared to that in control. Furthermore, ovarian tissues vitrified using this condition maintained follicle morphology and developmental capacity 9 weeks after grafting, as shown by an increased percentage of primary and secondary follicles and a significant decrease in the transition stage from primordial to primary stage follicles 5 and 9 weeks after grafting. In contrast, slow freezing and control groups lost intact follicles by 5 weeks after grafting. The described cryopreservation techniques, which preserve canine follicle development, will build the foundation of ovarian tissue cryopreservation to preserve female fertility in wild canids. |
| 巻・号 | 9(1) |
| ページ | 3970 |
| 公開日 | 2019-3-8 |
| DOI | 10.1038/s41598-019-40711-6 |
| PII | 10.1038/s41598-019-40711-6 |
| PMID | 30850725 |
| PMC | PMC6408471 |
| MeSH | Animals Cryopreservation / methods Cryoprotective Agents / pharmacology* Dogs Female Fertility Preservation / methods Freezing Ovarian Follicle / drug effects* Povidone / pharmacology* Tissue Culture Techniques / methods Vitrification |
| IF | 4.011 |
| 引用数 | 1 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ラット | F344 SCID rats |