論文 - 詳細
| RRC ID | 54517 |
|---|---|
| 著者 | Huber VJ, Igarashi H, Ueki S, Terumitsu-Tsujita M, Nito C, Ohno K, Suzuki Y, Itoh K, Kwee IL, Nakada T. |
| タイトル | Visualizing the Distribution of Matrix Metalloproteinases in Ischemic Brain Using In Vivo 19F-Magnetic Resonance Spectroscopic Imaging. |
| ジャーナル | Contrast Media Mol Imaging |
| Abstract |
Matrix metalloproteinases (MMPs) damage the neurovascular unit, promote the blood-brain barrier (BBB) disruption following ischemic stroke, and play essential roles in hemorrhagic transformation (HT), which is one of the most severe side effects of thrombolytic therapy. However, no biomarkers have presently been identified that can be used to track changes in the distribution of MMPs in the brain. Here, we developed a new 19F-molecular ligand, TGF-019, for visualizing the distribution of MMPs in vivo using 19F-magnetic resonance spectroscopic imaging (19F-MRSI). We demonstrated TGF-019 has sufficient sensitivity for the specific MMPs suspected in evoking HT during ischemic stroke, i.e., MMP2, MMP9, and MMP3. We then utilized it to assess those MMPs at 22 to 24 hours after experimental focal cerebral ischemia on MMP2-null mice, as well as wild-type mice with and without the systemic administration of the recombinant tissue plasminogen activator (rt-PA). The 19F-MRSI of TGN-019-administered mice showed high signal intensity within ischemic lesions that correlated with total MMP2 and MMP9 activity, which was confirmed by zymographic analysis of ischemic tissues. Based on the results of this study, 19F-MRSI following TGN-019 administration can be used to assess potential therapeutic strategies for ischemic stroke. |
| 巻・号 | 2019 |
| ページ | 8908943 |
| 公開日 | 2019-1-6 |
| DOI | 10.1155/2019/8908943 |
| PMID | 30723388 |
| PMC | PMC6339703 |
| MeSH | Animals Blood-Brain Barrier / diagnostic imaging Blood-Brain Barrier / metabolism Brain Ischemia / diagnostic imaging* Brain Ischemia / metabolism* Fluorine-19 Magnetic Resonance Imaging / methods* Male Matrix Metalloproteinase 2 / metabolism Matrix Metalloproteinase 3 / metabolism Matrix Metalloproteinase 9 / metabolism Matrix Metalloproteinases / metabolism* Mice Mice, Inbred C57BL Mice, Knockout |
| IF | 2.934 |
| 引用数 | 0 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 実験動物マウス | RBRC00398 |