RRC ID |
54546
|
Author |
Yamada S, Kimura M, Saito Y, Saito H.
|
Title |
Nrf2-mediated anti-oxidant effects contribute to suppression of non-alcoholic steatohepatitis-associated hepatocellular carcinoma in murine model.
|
Journal |
J Clin Biochem Nutr
|
Abstract |
The exact mechanisms of hepatocellular carcinoma development in non-alcoholic steatohepatitis remain unclear. In this study, we used a new class of high-fat diet, which could induce hepatocellular carcinoma development without the use of general chemical carcinogens or knockout mice. We investigated the correlation between hepatocellular carcinoma and oxidative stress/anti-oxidant effects after depletion of the gut microbiota by treatment with antibiotics. Mice fed with the steatohepatitis-inducing high-fat diet (STHD-01) for 41 weeks developed hepatocellular carcinoma. Antibiotic-treatment in mice fed with STHD-01 significantly depleted the gut microbiota and significantly ameliorated liver injury/histology. The tumor numbers of hepatocellular carcinoma were dramatically decreased by the antibiotics-treatment. We analyzed the factors involved in oxidative stress and anti-oxidant effects. Oxidative stress was elevated in mice fed with STHD-01, whereas some anti-oxidant factors were significantly elevated after antibiotics treatment. These results suggest that the gut microbiota is a key factor in improving oxidative stress induced by STHD-01 feeding.
|
Volume |
63(2)
|
Pages |
123-128
|
Published |
2018-9-1
|
DOI |
10.3164/jcbn.17-125
|
PII |
jcbn17-125
|
PMID |
30279623
|
PMC |
PMC6160730
|
IF |
2.19
|
Times Cited |
0
|
Resource |
DNA material |
Genomic DNA of Escherichia coli JCM 1649 T (JGD12168) |