Reference - Detail
| RRC ID | 54575 |
|---|---|
| Author | Shibuya K, Shirakawa J, Kameyama T, Honda S, Tahara-Hanaoka S, Miyamoto A, Onodera M, Sumida T, Nakauchi H, Miyoshi H, Shibuya A. |
| Title | CD226 (DNAM-1) is involved in lymphocyte function-associated antigen 1 costimulatory signal for naive T cell differentiation and proliferation. |
| Journal | J Exp Med |
| Abstract |
Upon antigen recognition by the T cell receptor, lymphocyte function-associated antigen 1 (LFA-1) physically associates with the leukocyte adhesion molecule CD226 (DNAM-1) and the protein tyrosine kinase Fyn. We show that lentiviral vector-mediated mutant (Y-F322) CD226 transferred into naive CD4+ helper T cells (Ths) inhibited interleukin (IL)-12-independent Th1 development initiated by CD3 and LFA-1 ligations. Moreover, proliferation induced by LFA-1 costimulatory signal was suppressed in mutant (Y-F322) CD226-transduced naive CD4+ and CD8+ T cells in the absence of IL-2. These results suggest that CD226 is involved in LFA-1-mediated costimulatory signals for triggering naive T cell differentiation and proliferation. We also demonstrate that although LFA-1, CD226, and Fyn are polarized at the immunological synapse upon stimulation with anti-CD3 in CD4+ and CD8+ T cells, lipid rafts are polarized in CD4+, but not CD8+, T cells. Moreover, proliferation initiated by LFA-1 costimulatory signal is suppressed by lipid raft disruption in CD4+, but not CD8+, T cells, suggesting that the LFA-1 costimulatory signal is independent of lipid rafts in CD8+ T cells. |
| Volume | 198(12) |
| Pages | 1829-39 |
| Published | 2003-12-15 |
| DOI | 10.1084/jem.20030958 |
| PII | jem.20030958 |
| PMID | 14676297 |
| PMC | PMC2194159 |
| MeSH | Antigens, Differentiation, T-Lymphocyte / physiology* Cell Differentiation Cell Division Gene Transfer, Horizontal Genetic Vectors Humans Interleukin-12 / physiology Interleukin-2 / pharmacology Lentivirus / genetics Lymphocyte Activation Lymphocyte Function-Associated Antigen-1 / physiology* Membrane Microdomains / physiology T-Lymphocytes / physiology* Th1 Cells / physiology |
| IF | 10.892 |
| Times Cited | 151 |
| Altmetric score |
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 17 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| DNA material | CS-CDF-CG-PRE (RDB04379) |